The characterization of female-specific gene fs 800 in Schistosoma

血吸虫女性特异性基因 fs 800 的表征

• 批准号: 03670194
• 负责人: AGATSUMA Takeshi
• 金额: $ 1.15万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670194/
• 关键词: Schistosoma mansoni; Female-specific gene; Chorion gene; fs 800 gene; fs800; 日本住血吸虫; DNA; 卵殻タンパク遺伝子; クロ-ニング

Egg production of worm pairs is a major cause of pathogenesis in schistosomiasis. Thus an understanding of female reproductive development, especially mechanisms of female-specific gene regulation is of major importance in disease control. A previously isolated cDNA that encodes the fs 800 protein was used to identify and isolate a recombinant phage containing an entire copy of the fs 800 gene. The gene is contained on a 5.5 kbp EcoRI fragment. There are no introns. The cap-site of the mRNA was determined by primer extension using RNA as template and dideoxy sequencing. The upstream regions of the gene contain putative cis-acting elements (TCACGT, GTAGAAT) that have been shown to play a role in the regulation of chorion gene expression in insects. The other elements that share a common core motif, TRRAAT(R=pyrimidine), are also found. These elements have been shown to bind nuclear proteins and control quantitative and qualitative changes in reporter gene expression in silkmoths.

寄生虫产卵是血吸虫病发病的主要原因。因此，了解女性生殖发育，特别是女性特异性基因调控机制在疾病控制中具有重要意义。使用先前分离的编码fs 800蛋白的cDNA来鉴定和分离含有fs 800基因的完整拷贝的重组噬菌体。该基因包含在5.5kbp的EcoRI片段上。没有内含子。通过使用RNA作为模板的引物延伸和双脱氧测序来确定mRNA的帽位点。该基因的上游区域含有推定的顺式作用元件（TCACGT，GTAGAAT），这些元件已被证明在昆虫绒毛膜基因表达的调节中发挥作用。另外还发现了具有相同核心基序TRRAAT（R=嘧啶）的其他元件，这些元件与家蚕核蛋白结合，控制报告基因表达的定量和定性变化。

项目成果

吾妻 健,P.T.Loverde: "マンソン住血吸虫卵形成に関与する雌性特異的遺伝子fs800のcharacterization" 寄生虫学雑誌. 40. 90 (1991)

Ken Azuma，P.T.Loverde：“曼氏血吸虫卵子发生中涉及的女性特异性基因 fs800 的特征”《寄生虫学杂志》40. 90 (1991)。

T.Agatsuma & P.T.Loverde: "The characterization of a Schistosoma mansom' femaleーspecific gene,fs800" Experimental Parasitology. (1992)

T.Agatsuma 和 P.T.Loverde：“血吸虫女性特异性基因的特征，fs800”实验寄生虫学（1992）。

T.Agatsuma: "Characterization of a Schistosoma mansoni female-specific gene (fs 800) involved in egg shell protein production." Jpn.J. Parasitol. 40. 90 (1991)

T.Agatsuma：“曼氏血吸虫女性特异性基因 (fs 800) 参与蛋壳蛋白生产的表征。”

T.Agatsuma & K.Terasaki: "Clonal diversity of triploids of the Japanese Fasciola species and relationships with other species of Fasciola" International Journal for Parasitology. (1992)

吾妻

吾妻 健: "マンソン住血吸虫卵形成に関与する雌性特異的遺伝子fs800のcharacterization" 寄生虫学雑誌. 40. 90 (1991)

Ken Azuma：“曼氏血吸虫卵子发生中涉及的女性特异性基因 fs800 的表征”《寄生虫学杂志》40. 90 (1991)。