Molecular phylogeny and Cytogenetics with reference to the origin of Schsitosoma japonicum

日本血吸虫起源的分子系统发育和细胞遗传学

• 批准号: 16406010
• 负责人: AGATSUMA Takeshi
• 金额: $ 8.38万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16406010/
• 关键词: Schistosoma japonicum; Schistosomatidae; Molecular phylogeny; Chromosome Microdissection; Genome mapping; Gene arrangements; Origin of Schistosoma; Coevolution; ミトコンドリアDNA; 核DNA; 染色体; 分子系藤樹; 分子進化; 種分化; 分子生物地理; 住血吸虫; 遺伝子配置; 肝臓超音波検査; Netwo

In this study, analyses for DNA sequences, mitochondrial (mt) DNA gene arrangements and chromosome mapping were carried out in order to verify the African origin theory of Schistosoma japonicum by Davis (1980, 1992). Our research to date reached the conclusion of the Asia origin theory, on the contrary. However, it is yet uncertain when and how 'Out of Asia' was possible for them to perform. It was also suggested from our studies that some members of the S. indicum group reintroduced from Africa to Asia, but it is also yet unknown about the rout and time of remigration. In this study, from the viewpoint of intra-specific variations, frequencies and geographic distributions of haplotypes in the CO1 region of the mt DNA were investigated in the S. indicum group. Two different haplotypes, that considerably differ from each other in sequence, were found in S. sindale from Bangladesh. The one from Bangladesh formed a cluster with that of S. sindale from Malaysia and Sri Lanka. The other fro … More m Bangladesh formed a cluster with that of S. sindale from Thailand. The difference in the DNA sequence between the two haplotypes corresponds with that between independent species. In addition, one of the hot regions (ND4-ND1) in the mt DNA gene arrangement of Schistosoma was analyzed for the first time using S. nasale, S. incognitum and S. bovis. As a result, it was clarified that these 3 species from Asia and Africa all turned out to be of the African type. A phylogenetic analysis using DNA sequences from each region as well as concatenated sequences including the three regions in the ND4-ND1 region plus the CO1 region was also carried out in order to confirm the taxonomical position of S. incognitum. The former analysis showed a rather unstable trees, but the latter revealed stable and congruent trees especially in terms of phylogenetic relationships between genera as well as species. Under these investigations, it was discovered that S. incognitum diverged earliest from the common ancestral species of the African group and the S. indicum group. Therefore, the result denies the idea that S. incognitum diverged from a common ancestor of the S. japonicum group. Less

本研究对日本血吸虫的DNA序列、线粒体DNA基因排列和染色体定位进行了分析，以验证Davis（1980，1992）的非洲起源学说。我们迄今的研究得出了亚洲起源论的结论，相反。然而，目前还不确定何时以及如何“走出亚洲”是可能的，他们的表现。我们的研究还表明，S. indicum组从非洲重新引入亚洲，但它也是未知的溃败和时间的重新迁移。本研究从种内变异的角度出发，对中国南方红豆杉线粒体DNA CO 1区单倍型的频率和地理分布进行了研究。indicum group.在S.来自孟加拉国的Sindale。孟加拉的一个与S.来自马来西亚和斯里兰卡的sindale。另一个Fro ...更多信息 m孟加拉国与S.来自泰国的Sindale。两种单倍型之间的DNA序列差异与独立物种之间的差异相对应。此外，首次利用S. nasale、黑脉草S. incognitum和S.牛结果表明，来自亚洲和非洲的这3种都是非洲类型。为了确定S的分类位置，还使用每个区域的DNA序列以及包括ND 4-ND 1区域中的三个区域和CO 1区域的串联序列进行了系统发育分析。无名氏前一种分析显示出一个相当不稳定的树，而后者显示出稳定和一致的树，特别是在属之间以及种之间的系统发育关系。在这些调查中发现，S。incognitum最早从非洲组和S. indicum group.因此，该结果否定了S. incognitum是从S. japonicum类群少

项目成果

輸入感染症、-保健微生物学・感染症学-スタンダード微生物学

输入性传染病，-健康微生物学/传染病-标准微生物学

• 发表时间: 2005
• 作者: Takeshi Agatsuma;R.P.V.J.Rajapakse;V.Y.Kuruwita;Moritoshi Iwagami;RC.Rajapakse;Takeshi Agatsuma;吾妻健
• 通讯作者: 吾妻健

Genotypic variation among lineages on Tiypanosoma cruz and its geographic aspects.

Tiypanosoma cruz 谱系间的基因型变异及其地理方面。

• 发表时间: 2004
• 期刊: Parasitology International 53(4)
• 作者: Higo H;Miura S;Horio M;Mimori T;Hamano S;Agatsuma T;Yanagi T;Cruz-Reyes A;Uyema N;Rojas A;Matta V;Akahane H;Hirayama K;Takeuchi T;Tada I;Himeno K
• 通讯作者: Himeno K

Studies on Morphology and Surface Topography of Bivitellobilharzia nairi in the Elephant in Sri Lanka

斯里兰卡大象体内Bivitellobilharzia nairi的形态和表面形貌研究

• 发表时间: 2005
• 期刊: J.Parasitology (accepted)
• 作者: R.P.VJ.Rajapakse;V.Y.Kuruwita;Moritoshi Iwagami;R.C.Rajapakse;Takeshi Agatsuma
• 通讯作者: Takeshi Agatsuma

Paragonimus skrjabini Chen, 1959 (Digenea: Paragonimidae) and related species in eastern Asia:: a combined molecular and morphological approach to identification and taxonomy

• DOI: 10.1007/s11230-004-1378-5
• 发表时间: 2005-01-01
• 期刊: SYSTEMATIC PARASITOLOGY
• 影响因子: 1.3
• 作者: Blair, D;Zhengshan, C;Ximei, Z
• 通讯作者: Ximei, Z

New filarial nematode from Japanese serows(Naemorhedus crispus : Bovidae) close to parasites from elephants.

来自日本羚羊（Naemorhedus Cripus：牛科）的新丝虫线虫与大象的寄生虫接近。

• 发表时间: 2006
• 期刊: Parasite 13・3
• 作者: Uni S;Bain O;Agatsuma T;Katsumi A;Baba M;Yanai T;Takaoka H
• 通讯作者: Takaoka H