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Studies on speciation of Asian schistosomes

亚洲血吸虫形态研究

基本信息

批准号：
05041098
负责人：
AGATSUMA Takeshi
金额：
$ 9.6万
依托单位：
Obihiro University of Agriculture and Veterinary Medicine (1994)Kochi Medical School (1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05041098/
关键词：
Phylogeny Schistosoma japonicum S.mekongi S.malaynsis Mitochondrial DNA PCR C-bannding pattern Chiasma Karyotype

项目摘要

Phylogeny among Asian Schistosoma flukes have not been known so far. In the present study, PCR technique was used to make up a molecular phylogeny on three Asian Schistosoma species, S.japonicum, S.mekongi, and S.malaynsis. A region of cytochrome C oxidase subunit I (COI) was amplified, and the fragment was cloned into TA-vector. The ABI sequencer/the Dye primer method were used. In total, 395 base pairs were sequenced, and analyzed with Paup (Maximum Parsimony Method) and Clustal V (The Neighbor-Joining Method). As outgroups, two African species, S.mansoni and S.haematobium were used. Both of the two methods constructed the same phylogenetic trees. That is, S.mekongi and S.malaynsis was clustered first, and joined with S.japonicum. The two African species were joined into a cluster, and bound with the Asian species.C-banding pattern analysis of the chromosomes was also performed The pattern showed closer relationships between S.mekongi and S.malaynsis, supporting the molecular phylogenetic tree obtained in this study. Karyotypic analysis of the snail host species, Robertsiella gismani, Neotricula aperta, and Oncomelania nosophora were carried out. It was found that chromosome numbers were all 2n=34, but sex chromosomes seem to be of XY type and XO type for Robertsiella gismani and Neotricula aperta, respectively. In the case of Oncomelania nosophora, no sex chromosome differenciation occurred.Infection experiments were also carried out. Three Japanese snails, Oncomelania nosophora, O.minima and Bythinella nipponica were used. It was found that only Oncomelania nosophora was susceptible to S.japonicum and S.malaynsis infection.

迄今为止，亚洲血吸虫的系统发育尚不清楚。本研究采用PCR技术对日本血吸虫、米孔血吸虫和马来西亚血吸虫三种亚洲血吸虫进行了分子系统发育分析。扩增细胞色素C氧化酶I亚基（COI）片段，并将其克隆到ta载体上。采用ABI测序仪/染料引物法。共测序395个碱基对，并采用Paup （Maximum Parsimony Method）和cluster V （The Neighbor-Joining Method）进行分析。作为外群，使用了两个非洲种，S.mansoni和S.haematobium。两种方法构建了相同的系统发育树。也就是说，m. kongi和m. malaynsis首先聚集在一起，然后与S.japonicum合并。将这两个非洲种与亚洲种结合成一个簇，并进行了染色体的c带模式分析，该模式显示了S.mekongi和S.malaynsis之间更密切的关系，支持了本研究得到的分子系统发育树。对三种寄主螺（Robertsiella gismani, Neotricula aperta, Oncomelania nosophora）进行了核型分析。结果发现，吉斯马尼Robertsiella gismani和新三角藻（Neotricula aperta）的性染色体数目均为2n=34，但性染色体似乎分别为XY型和XO型。在嗜黑钉螺的情况下，没有发生性染色体分化。同时进行了感染实验。选用日本钉螺3只，分别为钉螺、小钉螺和日本血吸虫。结果发现，只有医院钉螺对日本血吸虫和马来西亚血吸虫感染敏感。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

吾妻健、柳从笑、田口尚弘、平井啓久、波部重久、平田瑞城、K.ライ、S.ウパタム: "ミトコンドリアDNAから見たアジア産日本住血吸虫類の系統類縁関係" 第41回日本寄生虫学会、日本衛生動物学会北日本支部合同大会講演要旨. 18 (1994)

Ken Azuma、Keisho Yanagi、Naohiro Taguchi、Hirohisa Hirai、Shigehisa Nabe、Mizuki Hirata、K. Lai、S. Upatham：“从线粒体 DNA 观察到的亚洲日本血吸虫的系统发育关系”第 41 届日本寄生主义联合会议摘要昆虫学会和日本卫生动物学会北日本分会 18 (1994)。

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Taguchi T., Hirai H., Habe S., Hirata M., Kawanaka M., Lai K., Upatham E.S.& Agatsuma T.: "Comparative karyotype study of snail hosts of Oriental schistosomes" Japanese Journal of Parasitology. 44 (Suppl.). 127- (1995)

田口 T.、平井 H.、哈贝 S.、平田 M.、川中 M.、赖 K.、乌帕瑟姆 E.S.

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Agatsuma T., Liu C.X., Taguchi T., Matsuoka M., Hirai H., Habe S., Hirata M.& Lai K.: "Phylogenetic relationship between Malaysian and Japanese Schistosoma species from a view point of mitochondrial DNA sequences" Japanese Journal of Parasitology. 43 (Sup

Agatsuma T.，刘C.X.，田口T.，松冈M.，平井H.，Habe S.，平田M.

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吾妻健、柳从笑、田口尚弘、松岡未央、平井啓久、波部重久、平田瑞城、K.ライ: "ミトコンドリアDNAから見たマレーシア住血吸虫と日本住血吸虫の系統類縁関係" 寄生虫学雑誌. 43(Suppl.). 86 (1994)

Ken Azuma、Sho Yanagi、Naohiro Taguchi、Mio Matsuoka、Hirohisa Hirai、Shigehisa Nabe、Mizuki Hirata、K. Lai：“从线粒体 DNA 观察马来西亚血吸虫和日本血吸虫之间的系统发育关系”寄生虫学杂志 43（增刊）。 86 (1994)