Analysis of simultaneous allotransplantation of small intestine and liver

第一个系列的实验检验了肌内注射FK506对同种异体小肠移植物存活的影响。DA和LEW大鼠分别作为小肠供体和受体。移植后给予FK506 （0.3mg/kg、1.0mg/kg或2mg/kg）腹腔注射14天。结果清楚地表明，每天2.0mg/kg剂量的FK506可以抑制同种异体小肠移植的排斥反应，而作为该药物的并发症，所有大鼠死于肺炎。为了减少同种异体小肠移植对FK506的高剂量需求，我们尝试探讨同种异体小肠和肝脏同时移植的效果。然而，接受者死于感染。我们改变方案来分析供体特异性输血（DST）和FK506对小肠同种异体移植的影响。受体（LEW）在第-8天（第0天）接受1.5 ml新鲜抽取的DA iv血。FK506从第8天至第4天以每天1.0mg/kg的剂量给药，从第0天至第14天以每天0.3mg/kg的剂量给药。结果清楚地表明，FK506联合DST可有效预防急性排斥反应。DST联合低剂量FK506可获得长期的同种异体移植物存活。为了检测DST+FK506对T细胞功能的体内影响，我们检测了DST+FK506处理大鼠的CTL和IL-2的产生。结果表明，DST+FK506可选择性抑制抗da t细胞反应，而不抑制t细胞对第三方异体抗原的反应。

The first series of experiments examined the effect of intra-muscular(im) administration of FK506 on small intesitine allo-graft survival. DA and LEW rats were used as small intestine donors and recipients. FK506 (0.3mg/kg, 1.0mg/kg or 2mg/kg) was given im for 14 days after transplantation. The results clearly demonstrated that the rejection of small intestine allograft was suppressed by FK506 at the dosage of 2.0mg/kg per day, whereas, as a complication of this drugs, all rats died of pneumonia. In order to diminish the requirements for high doses of FK506 in recipients of small intestine allograft, we tried to investigate the effect simultaneous allotransplantation of small intestine and liver. However, recipients died of infection. We changed the protocol to analyze the effect of donor-specific transfusion (DST) and FK506 on small intestine allotransplantation. Recipients (LEW) received 1.5 ml of freshly drawn DA blood iv on day -8 with respect to grafting ( day 0 ). FK506 was administered at a dose of 1.0mg/kg per day from day -8 to day -4, and at 0.3mg/kg per day from day 0 to day +14. The results clearly demonstrated that FK506 combined with DST is effective to prevent acute rejection. Longterm allograft survival could be obtained by conditioning with combined DST and low-dose FK506. To test for in vivo effects of DST+FK506 on T cell function , CTL and IL-2 production were tested in rats treated with DST+FK506. The results suggested that DST+FK506 resulted in selective inhibition of anti-DA T-cell responses without suppressing T-cell responses to third party alloantigens.