Expression of adhesion molecules and VEGF in Childhood Malignant Solid Tumors

粘附分子和VEGF在儿童恶性肿瘤中的表达

• 批准号: 11671776
• 负责人: FUKUZAWA Masahiro
• 金额: $ 2.3万
• 依托单位: Nihon University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671776/
• 关键词: CD44; neuroblastoma; VEGF; Flk-1; angiogenesis; VEGF; Flk-1; カドヘリン; VLA-4

While angiogertic factors may play an important role in the biology of neuroblastoma, which frequently spreads hematogenously. the mechanism of spread remains unclear. We studied tumor progression and invasion from the perspective of angiogenesis, and sought to understand the features of this type of tumor. Thirty-one specimens were resected from patient with neuroblastoma treated at our institution from 1988 to 1998 and the expressions of VEGF and its receptor (Flk-1) were examined using immunohistochemistry. Staining was performed using anti-VEGF monoclonal antibody and Flk-1 polyclonal antibody. A microvessel deteccion procedure was performed using anti-Factor VIII-related antigen. We looked for correlations among the expressions of VEGF and its receptors, microvessel density, and various clinicopathologic factors. In addition, we examined the expression and location of VEGF mRNA and Flk-1 mRNA in 10 primary neuroblastomas resected from 1998 to 1999, using in situ hybridizacion. Both in situ hybridization and immunohistochemistry demonstrated the presence of VEGF expression within the neuroblastoma cells. We flound VEGF mRNA in neuroblastoma cells but not vascular endothelial cells, according to in situ hybridization. Further, FlK-1 mRNA is present both in neuroblastoma cells and vascular endothelial cells, according to in situ hybridization. The level of VEGF expression is higher in unfavorable histology using the criteria of Shimada than in favorable histology. We suggested that paracrine and autocrine systems are involved in the antgiogenesis of neuroblastoma and that the expression of VEGF correlates with the prognosis in neuroblastoma.

而血管生成因子可能在神经母细胞瘤的生物学中起重要作用，神经母细胞瘤经常通过血液传播。传播机制尚不清楚。我们从血管生成的角度研究了肿瘤的进展和侵袭，并试图了解这类肿瘤的特点。应用免疫组织化学方法检测31例神经母细胞瘤标本中VEGF及其受体（Flk-1）的表达。使用抗VEGF单克隆抗体和Flk-1多克隆抗体进行染色。用抗凝血因子VIII相关抗原进行微血管检测。我们寻找VEGF及其受体表达、微血管密度和各种临床病理因素之间的相关性。此外，我们还应用原位杂交技术检测了1998 ~ 1999年切除的10例原发性神经母细胞瘤中VEGF mRNA和Flk-1 mRNA的表达和定位。原位杂交和免疫组化均显示神经母细胞瘤细胞内存在VEGF表达。原位杂交结果显示，VEGF mRNA在神经母细胞瘤细胞中表达，而在血管内皮细胞中无表达。此外，根据原位杂交，FlK-1 mRNA存在于神经母细胞瘤细胞和血管内皮细胞中。VEGF表达水平在使用Shimada标准的不良组织学中高于良好组织学。提示旁分泌和自分泌系统参与了神经母细胞瘤的血管生成，VEGF的表达与神经母细胞瘤的预后有关。

项目成果

Sugiura H, Fukuzawa M.: "Expression and localization of VEGF (vascular endothelial growth factor) and its receptors in human neuroblastoma."J.Nihon Univ.Med.Ass.. 59. 578-585 (2000)

Sugiura H、Fukuzawa M.：“人神经母细胞瘤中 VEGF（血管内皮生长因子）及其受体的表达和定位。”J.Nihon Univ.Med.Ass.. 59. 578-585 (2000)

杉浦浩朗,福澤正洋: "神経芽腫におけるVEGFおよび、そのレセプターの発現と局在に関する検討"日大医学雑誌. 59・12. 578-585 (2000)

Hiroaki Sugiura、Masahiro Fukuzawa：“神经母细胞瘤中 VEGF 及其受体的表达和定位的研究”日本大学医学杂志 59・12（2000）。