Basic Study for Autologous Vaccine Therapy for Neuroblastoma

神经母细胞瘤自体疫苗治疗的基础研究

• 批准号: 15591894
• 负责人: FUKUZAWA Masahiro
• 金额: $ 2.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591894/
• 关键词: Neuroblastoma; CpG-ODN; apoptosis; GM-CSF; Vaccine Therapy; GM-CS

The aim of the study was to clarify the anti-tumor effect of combination vaccine therapy using irradiated autologous neuroblastoma and CpG oligonucleotide with or without GM-CSF which activates dendritic cells, in a mouse model. Also we investigated the effect of this vaccine therapy to minimal residual disease. We inoculated weakly-immunogenic Neuro-2a cells in syngeneic A/J mouse. After resection of the subcutaneous tumor mass, these cells were prepared by irradiation and then used for vaccination. CpG ODN and GM-CSF producing B78H1 cells were injected together with the vaccination. Tumor growth in the vaccination after surgery group was significantly suppressed relative to the control, surgery alone, and vaccination alone groups. Survival was also significantly improved in the vaccination after surgery group.Although the production of IFN-γ in spleen cells was suppressed by the combination vaccine therapy in a mouse with huge tumor, the production was activated in a mouse whose tumor had been resected. Thus, this combination vaccine therapy was more effective in a mouse with minimal residual disease than in a mouse with huge tumor.

该研究的目的是在小鼠模型中阐明联合疫苗疗法的抗肿瘤效果，该联合疫苗疗法使用经辐照的自体神经母细胞瘤和 CpG 寡核苷酸（加或不加 GM-CSF）来激活树突状细胞。我们还研究了这种疫苗疗法对微小残留病的影响。我们在同系 A/J 小鼠中接种了弱免疫原性 Neuro-2a 细胞。切除皮下肿瘤块后，通过照射制备这些细胞，然后用于疫苗接种。与疫苗接种一起注射产生CpG ODN和GM-CSF的B78H1细胞。相对于对照组、单纯手术组和单纯疫苗接种组，术后疫苗接种组的肿瘤生长被显着抑制。手术后接种疫苗组的存活率也显着提高。虽然在患有巨大肿瘤的小鼠中，联合疫苗疗法抑制了脾细胞中IFN-γ的产生，但在肿瘤被切除的小鼠中，该产生被激活。因此，这种联合疫苗疗法对具有微小残留疾病的小鼠比对具有巨大肿瘤的小鼠更有效。

项目成果

マウス神経芽腫におけるCpG oligonucleotideを用いたGM-CSF併用抗腫瘍ワクチン療法の検討

使用 CpG 寡核苷酸对小鼠神经母细胞瘤进行 GM-CSF 联合抗肿瘤疫苗治疗的检验

• 发表时间: 2003
• 期刊: 日大医学雑誌 62巻11号
• 作者: 小林 元;柴田昌彦;福澤正洋
• 通讯作者: 福澤正洋

CpG Oligonucleotides-Combined Autologous Vaccine Therapy using GM-CSF is Effective for Post-Surgical Recurrence of Mouse Neuroblastoma

使用 GM-CSF 的 CpG 寡核苷酸联合自体疫苗治疗可有效治疗小鼠神经母细胞瘤术后复发

• 发表时间: 2003
• 期刊: J.Nihon Univ.Med.Ass. 62(11)
• 作者: Kobayashi G;Shibata M;Fukuzawa M;Sandler AD
• 通讯作者: Sandler AD