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A Study of Growth Factor in Prostate Cancer : Effects of Bombesin and its Antibody on Growth of Human Prostate Carcinoma Cell Lines

前列腺癌生长因子的研究：铃蟾肽及其抗体对人前列腺癌细胞系生长的影响

基本信息

批准号：
03670743
负责人：
HARADA Tadashi
金额：
$ 1.34万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670743/
关键词：
Prostate cancer Growth factor Bombesin GRP Autocrine growth factor

项目摘要

Bombesin(BMBS), a tetradecapeptide extracted from frog skin, was evaluated with its antibody in vitro and in vivo to evaluate growth modulating effects on the DU-145 and PC-3 human prostate carcinoma cell line. The two cell lines were maintained in DME medium containing 2% fetal calf serum and 1 mg/ml insulin in a 37^oC humidified atmosphere of 5% CO_2 and 95% air. BMBS caused a definite effect on a dose-dependent increase in DU-145 and PC-3 cell growth. The addition of antibody to BMBS, called Ab-BMBS rabbit antiserum during the lag phase of growth, resulted in the specific inhibition of DU-145 and PC-3 growth, with or without BMBS.In in vivo experiments, nude mice were implanted with DU-145 cells in order to evaluate the suppressing effects of Ab-BMBS on carcinoma cells. After the inoculation of 107 DU-145 cells into each mouse, 20mg Ab-BMBS was intraperitoneally injected into the each mouse three times weekly, for three weeks. In mice administered with Ab-BMBS, the growth of DU-145 was markedly suppressed. By immunocytochemical study, BMBS immuno-reactivities were detected on DU-145 and PC-3 cells.These results suggest that BMBS can function as an autocrine growth factor for human prostate carcinoma cells. Furthermore, as the result of xenografts in mice, we have learned that Ab-BMBS may inhibit the growth of human prostate carcinoma.

蛙皮肽（Bombesin，BMBS）是一种从蛙皮中提取的十四肽，用其抗体在体外和体内评价其对DU-145和PC-3人前列腺癌细胞系的生长调节作用。将两株细胞培养在含2%胎牛血清和1 mg/ml胰岛素的DME培养基中，在37 ℃、5%CO_2和95%空气的潮湿环境中。BMBS对DU-145和PC-3细胞生长的剂量依赖性增加产生一定的影响。在生长停滞期加入抗BMBS的兔抗血清（Ab-BMBS兔抗血清），可特异性抑制DU-145和PC-3细胞的生长，并在裸鼠体内接种DU-145细胞，观察Ab-BMBS对肿瘤细胞的抑制作用。每只小鼠接种107个DU-145细胞后，腹腔注射Ab-BMBS 20 mg，每周3次，共3周。在施用Ab-BMBS的小鼠中，DU-145的生长被显著抑制。用免疫细胞化学方法检测BMBS在DU-145和PC-3细胞上的免疫反应性，结果表明BMBS对人前列腺癌细胞具有自分泌生长因子的作用。此外，作为小鼠异种移植的结果，我们已经了解到Ab-BMBS可以抑制人前列腺癌的生长。