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Molecular biological study of the latent viral infection of the inner ear

内耳潜伏病毒感染的分子生物学研究

基本信息

批准号：
03670803
负责人：
FUKUDA Satoshi
金额：
$ 1.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670803/
关键词：
Herpes simplex virus Varicella-zoster virus Latent infection Human geniculate ganglia Human spiral ganglia Human vestibular ganglia Polymerase chain reaction (PCR)in situ hybridization (ISH)ウイルス潜伏感染内耳 HSVー1 ラセン神経節 in situ hybriz

项目摘要

Viral infection, especially by reactivation of latent herpes virus has been considered to be a possible explanation for the pathogenesis of idiopathic peripheral facial nerve palsy (Bell's palsy),sudden sensorineural hearing loss,vestibular neuronitis and Ramsey-Hunt syndrome. As of yet,however, latent HSV-1 and VZV in the sensory neurons of the geniculate, spiral or vestibular ganglia has been sitll unknown.In this project,we examined the HSV type 1(HSV-1) and varicella-zoster virus DNA in human geniculate, spiral and vestibular ganglia of autopsy patients by using molecular bioological technique, polymerase chain reaction(PCR), in situ hybridization (ISH) and reverse transcrioption PCR(RT-PCR) methods.The summary of the results are as follows;1) We detected HSV-1 DNA in 15 of 17 geniculate ganglia, in 10 of 10 spiral ganglia and in 6 of 10 vestibular ganglia by PCR method.2) HSV-1 latency associated transcript (LAT) was detected in 11 of 15 geniculate ganglia, in none of 10 spiral and vestibular ganglia.by ISH method.3) By usingn more sensitive RT-PCR, we detected HSV-1 LAT in all of 8 geniculate ganglia examined,in 2 of 8 vestibular ganglia and none of 8 spiral ganglia.4) We also detected VZV DNA in 9 of 13 geniculate ganglia and 2 of 10 spiral and vestibular ganglia.by PCR method.These results suggest that latent HSV-1 and VZV in the human geniculate, spiral and vestibular ganglia may possible causes of idiopathic peripheral facial nerve palsy ( Bell's palsy ),sudden sensorineural hearing loss,vestibular neuronitis and Ramsey-Hunt syndrome. However, the absence of the HSV-1 LAT in spiral ganglia in particular indicates the incidence of HSV-1 reactivation of the spiral ganglia may be rare.

病毒感染，特别是潜伏的疱疹病毒的再激活被认为是特发性周围面神经麻痹（贝尔麻痹），突发性感音神经性听力损失，前庭神经元炎和拉姆齐-亨特综合征的发病机制的一个可能的解释。本研究应用分子生物学技术、聚合酶链反应（PCR）、免疫组化技术和免疫组化技术，检测了人膝状体神经节、螺旋神经节和前庭神经节中HSV-1和水痘-带状疱疹病毒（VZV）的DNA，并对HSV-1和VZV在人膝状体神经节、螺旋神经节和前庭神经节中的分布进行了研究。原位杂交（ISH）和逆转录PCR（RT-PCR）方法。1）在17个膝状神经节中，15个检出HSV-1DNA，2）15个膝状体神经节中有11个检测到HSV-1潜伏期相关转录本（LAT），10例螺旋和前庭神经节均未ganglia.by1 LAT。3）采用更敏感的RT-PCR方法，在8例膝状神经节中均检出HSV-1 LAT，前庭神经节2例，螺旋神经节0例。用PCR方法在13个膝状体神经节中的9个和10个螺旋神经节和前庭神经节中的2个中检测到VZV DNA，结果表明，人膝状体神经节中存在潜伏的HSV-1和VZV，螺旋神经节和前庭神经节可能是特发性周围性面神经麻痹（贝尔麻痹）、突发性感音神经性耳聋、前庭神经元炎和Ramsey-Hunt综合征的病因。然而，螺旋神经节中HSV-1 LAT的缺失特别表明螺旋神经节的HSV-1再激活的发生率可能是罕见的。