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Molecular biological study of the latent viral infection and its reactivation of the human sensory ganglia in the temporal bone.

潜伏病毒感染及其对颞骨中人类感觉神经节重新激活的分子生物学研究。

基本信息

批准号：
05671411
负责人：
FUKUDA Satoshi
金额：
$ 1.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Viral infection, in particular, by reactivation of latent herpes virus has been considered to be a possible explanation for the pathogenesis of idiopathic peripheral facial nerve palsy (Bell's palsy), sudden sensorineural hearing loss, vestibular neuronitis and Ramsey-Hunt syndrome. As of yet, however, latent HSV-1 and VZV in the sensory neurons of the geniculate, spiral or vestibular ganglia and the way for reactivation has been sitll unknown.Up to now, although we could obviously detect latency-associated transcript (LAT) in trigeminal and geniculte ganglia, only one vestibular ganglia showed positive for LAT and negative in spiral ganglia. We postulated that the difference of infected copy number between these ganglia might influence these results. To elucidate this problem, in this project, we further examined these ganglia using molecular biological technique. A relative amount of LAT between each ganglia were calculated. HSV-1 DNA or copy number of LAT in trigeminal and geniculate ganglia were 10-100 times as many as taht of vbestibular ganglia. The percentage of positive hybridization number for LAT out of total ganglia calculated was 1.2% in trigeminal ganglion and 6.2% in geniculate ganglion. On the other hand, the positive rate in vestibular ganglion was under 0.1% and spiral ganglia showed no positive findings. Our results suggest the neurotropism of HSV-1 may different in each sensory ganglia, but the difference of positive infectid number for LAT between each sensory ganglia remains still nuclear.

病毒感染，特别是通过潜伏疱疹病毒的重新激活，被认为是特发性周围性面神经麻痹（贝尔麻痹）、突发感音神经性听力损失、前庭神经元炎和拉姆齐亨特综合征发病机制的可能解释。然而，迄今为止，膝状神经节、螺旋神经节或前庭神经节的感觉神经元中潜伏的HSV-1和VZV以及重新激活的方式尚不清楚。到目前为止，虽然我们可以在三叉神经节和膝状神经节中明显检测到潜伏相关转录物（LAT），但只有一个前庭神经节呈LAT阳性，螺旋神经节呈阴性。我们假设这些神经节之间感染拷贝数的差异可能会影响这些结果。为了阐明这个问题，在这个项目中，我们利用分子生物学技术进一步检查了这些神经节。计算每个神经节之间 LAT 的相对量。三叉神经节和膝状神经节中的HSV-1 DNA或LAT拷贝数是前庭神经节的10-100倍。计算出LAT阳性杂交数占总神经节的百分比，三叉神经节为1.2%，膝状神经节为6.2%。另一方面，前庭神经节的阳性率低于0.1%，螺旋神经节未见阳性发现。我们的结果表明HSV-1的神经趋向性在每个感觉神经节中可能不同，但每个感觉神经节之间LAT阳性感染数量的差异仍然是核的。