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Experimental Study of Possible Immunological Role of Endolymphatic Sac in Inner Ear Viral Infection

内淋巴囊在内耳病毒感染中可能的免疫学作用的实验研究

基本信息

批准号：
63570803
负责人：
FUKUDA Satoshi
金额：
$ 1.41万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570803/
关键词：
Guinea Pig Cytomegalovirus (GPCMV)Endolymphatic sac Spiral modiolar vein Inner ear immune response Viral infection of the inner ear Guinea Pig Cytomegalovirus(GPCMV)Guinea Pig Specific Cytomegalovirus(GPCMV)Action Potential(AP)

项目摘要

The endolymphatic sac and cochlea including spiral modiolar vein were investigated in the guinea pig inoculated Guinea Pig Cytomegalovirus (GPCMV) and psoralen-ultraviolet inactivated GPCMV into the cochlea of seronegative and seropositive animal.Histopathological including immunoelectron microscopic study and electophysiological investigation were carried out at day 2, 4, 6, 8, to elucidate the possible immunological role of endolymphatic sac and cochlea in inner ear viral infection.The summary of results are as follows ;1) Hearing loss was found in the seronegative group whereas hearing was protected in the seropositive and control group.2) It was recognized that inflammatory cells infiltration via collecting venule and spiral modiolar vein from systemic circulation actually occurs in the GPCMV inoculation to the cochlea of seronegative group.3) No inflammatory cells infiltration was recognized in the cochlea including spiral modiolar vein of seropositive and control group.4) Only slight inflammatory cells infiltration was found in the endolymphatic sac in this system. But endolymphatic sac reacts immunologically with direct inoculation of GPCMV into the endolymphatic sac.5) There must be 2 system to react to the stimulation of virus inoculation to the cochlea. Namely, spiral modiolar vein of the cochlea and endolymphatic sac. The degree how these area reacts to the viral infection in the inner ear is still controversial and wait for further precise time cours e study in this particular area.

将豚鼠巨细胞病毒(GPCMV)和补骨脂素紫外线灭活的GPCMV分别接种于血清阴性和血清阳性豚鼠的耳蜗内，分别于感染后第2、4、6、8天进行组织病理学包括免疫电子显微镜和电生理学检查，以探讨内淋巴囊和耳蜗囊在内耳病毒感染中可能的免疫学作用。1)血清阴性组大鼠听力受损，而血清阳性组和对照组大鼠听力得到保护。2)GPCMV免疫后，血清阴性组耳蜗炎性细胞通过收集小静脉和螺旋耳缘静脉进入耳蜗腔。3)血清阴性组耳蜗炎性细胞未见。而内淋巴囊对GPCMV的免疫反应是直接接种到内淋巴囊中的。5)内淋巴囊必须有两个系统对病毒接种耳蜗所产生的刺激反应。即耳蜗线上静脉和内淋巴囊。这些区域对内耳病毒感染的反应程度仍然存在争议，并等待这一特定区域的进一步确切时间过程研究。