Experimental Study of Possible Immunological Role of Endolymphatic Sac in Inner Ear Viral Infection

将豚鼠巨细胞病毒（GPCMV）和经紫外线灭活的GPCMV分别接种于血清阴性和血清阳性动物的耳蜗，观察豚鼠耳蜗内淋巴囊和耳蜗包括螺旋蜗轴静脉的形态学变化，并于接种后第2、4、6、8、12、14、16、18、19探讨内淋巴囊和耳蜗在内耳病毒感染中的免疫学作用。1）听力损失在血清阴性组中发现，而听力在血清阳性组和对照组中得到保护。血清阴性组耳蜗接种GPCMV后，炎症细胞从体循环经集合小静脉和螺旋蜗轴静脉浸润。血清阳性组和对照组耳蜗内包括蜗轴静脉内均未见炎性细胞浸润。4）内淋巴囊内仅见轻度炎性细胞浸润。而GPCMV直接接种内淋巴囊后，内淋巴囊发生免疫反应。5）耳蜗对病毒接种刺激的反应必须有两个系统。即耳蜗螺旋蜗轴静脉和内淋巴囊。这些区域对内耳中病毒感染的反应程度仍然存在争议，并等待在该特定区域进一步精确的时间过程研究。

The endolymphatic sac and cochlea including spiral modiolar vein were investigated in the guinea pig inoculated Guinea Pig Cytomegalovirus (GPCMV) and psoralen-ultraviolet inactivated GPCMV into the cochlea of seronegative and seropositive animal.Histopathological including immunoelectron microscopic study and electophysiological investigation were carried out at day 2, 4, 6, 8, to elucidate the possible immunological role of endolymphatic sac and cochlea in inner ear viral infection.The summary of results are as follows ;1) Hearing loss was found in the seronegative group whereas hearing was protected in the seropositive and control group.2) It was recognized that inflammatory cells infiltration via collecting venule and spiral modiolar vein from systemic circulation actually occurs in the GPCMV inoculation to the cochlea of seronegative group.3) No inflammatory cells infiltration was recognized in the cochlea including spiral modiolar vein of seropositive and control group.4) Only slight inflammatory cells infiltration was found in the endolymphatic sac in this system. But endolymphatic sac reacts immunologically with direct inoculation of GPCMV into the endolymphatic sac.5) There must be 2 system to react to the stimulation of virus inoculation to the cochlea. Namely, spiral modiolar vein of the cochlea and endolymphatic sac. The degree how these area reacts to the viral infection in the inner ear is still controversial and wait for further precise time cours e study in this particular area.