Experimental Study of Possible Immunological Role of Endolymphatic Sac in Inner Ear Viral Infection

在豚鼠接种豚鼠巨细胞病毒（GPCMV）和补骨脂素紫外线灭活的 GPCMV 到血清阴性和血清阳性动物的耳蜗中，对内淋巴囊和耳蜗（包括螺旋蜗轴静脉）进行了研究。在第 2、4、6、8 天进行了组织病理学包括免疫电镜研究和电生理学研究，以阐明可能的情况内淋巴囊和耳蜗在内耳病毒感染中的免疫学作用。结果总结如下；1）血清阴性组出现听力损失，而血清阳性和对照组听力得到保护。2）认识到，在GPCMV接种到血清阴性组的耳蜗中，实际上发生了来自体循环的集合小静脉和螺旋蜗轴静脉的炎症细胞浸润。3）在血清阴性组的耳蜗中未发现炎症细胞浸润。血清阳性组和对照组的耳蜗包括螺旋蜗轴静脉。4)该系统的内淋巴囊中仅发现轻微的炎性细胞浸润。但内淋巴囊会因将GPCMV直接接种到内淋巴囊而发生免疫反应。5)必须有2个系统对病毒接种到耳蜗的刺激作出反应。即耳蜗和内淋巴囊的螺旋蜗轴静脉。这些区域对内耳病毒感染的反应程度仍然存在争议，需要等待该特定区域的进一步精确研究。

The endolymphatic sac and cochlea including spiral modiolar vein were investigated in the guinea pig inoculated Guinea Pig Cytomegalovirus (GPCMV) and psoralen-ultraviolet inactivated GPCMV into the cochlea of seronegative and seropositive animal.Histopathological including immunoelectron microscopic study and electophysiological investigation were carried out at day 2, 4, 6, 8, to elucidate the possible immunological role of endolymphatic sac and cochlea in inner ear viral infection.The summary of results are as follows ;1) Hearing loss was found in the seronegative group whereas hearing was protected in the seropositive and control group.2) It was recognized that inflammatory cells infiltration via collecting venule and spiral modiolar vein from systemic circulation actually occurs in the GPCMV inoculation to the cochlea of seronegative group.3) No inflammatory cells infiltration was recognized in the cochlea including spiral modiolar vein of seropositive and control group.4) Only slight inflammatory cells infiltration was found in the endolymphatic sac in this system. But endolymphatic sac reacts immunologically with direct inoculation of GPCMV into the endolymphatic sac.5) There must be 2 system to react to the stimulation of virus inoculation to the cochlea. Namely, spiral modiolar vein of the cochlea and endolymphatic sac. The degree how these area reacts to the viral infection in the inner ear is still controversial and wait for further precise time cours e study in this particular area.