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Latent Viral infection of the inner ear

内耳潜伏性病毒感染

基本信息

批准号：
11671666
负责人：
FUKUDA Satoshi
金额：
$ 2.24万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671666/
关键词：
Latent viral infection Polymerase chain reaction (PCR)Herpes simplex virus VZV HHV-6 Human geniculate ganglia Human spiral ganglia Human vestibular ganglia

项目摘要

Because we could not get enough number of autopsy specimen of sensory ganglia within temporal bone, we focused on 3points as follows.1) A case of sudden deafness with early detection of salivary VZV DNA.-Recovery by treatment of combination therapy with anti-viral agent-. We report a case of sudden deafness with detection of VZV DNA from saliva by PCR.Anti-viral drug was considered to be effective for this case. Sixty-nine year-old, female noticed right sudden hearing loss. We detected VZV DNA from her saliva on day-2. She diagnosed right sudden sensory neural hearing loss and treated with steroid and also anti-viral agent. Her hearing recovered by this treatment strategy. We detected VZV DNA from her saliva by PCR, but no rise in titer of serum anti-VZV antibody. It is suggested that anti-viral drug is effective for sudden sensory neural hearing loss in such case with detection of viral DNA from saliva by PCR.2) RT-PCR amplification of viral RNA from celloidin-embedded human temporal bone sections. The aim of this study was to investigate whether it is possible to detect viral RNA from celloidin-embedded fetal temporal bone with microscopic abnormality. Sections with microscopic abnormality were selected and cut into small pieces. RNA was extracted from the tissue and purified. Reverse transcription was utilized for cDNA synthesis. DNA was amplified in nested-PCR.Though We could not detect Rubella RNA extracted from celloidin-embedded fetal temporal bone. Alpha-tubulin RNA was detected 8 out of 11 specimen (72.8%). These results indicate that RNA can be analyzed from archival celloidin-embedded human temporal bone sections.3) We could detect HHV-6 DNA from 1 trigeminal ganglia specimen of 6 specimen. This result may indicate that HHV-6 may cause some otologic diseases by reactivation. We will have to wait for further study in this particular area in increased number.

由于我们无法获得足够数量的颞骨内感觉神经节的尸检标本，我们重点关注以下三点。1）早期检测唾液VZV DNA的突发性耳聋病例。-通过抗病毒药物联合治疗恢复-。我们报告一例突发性耳聋病例，通过PCR从唾液中检测到VZV DNA。抗病毒药物被认为对该病例有效。六十九岁，女性，突发性耳聋。我们在第二天从她的唾液中检测到了 VZV DNA。她诊断出患有突发性感觉神经性听力损失，并接受类固醇和抗病毒药物治疗。通过这种治疗策略，她的听力恢复了。我们通过 PCR 从她的唾液中检测到了 VZV DNA，但血清抗 VZV 抗体滴度没有升高。通过PCR检测唾液中的病毒DNA，表明抗病毒药物对于突发性感觉神经性听力损失是有效的。2）对赛璐珞包埋的人颞骨切片中的病毒RNA进行RT-PCR扩增。本研究的目的是探讨是否有可能从具有微观异常的赛璐珞包埋的胎儿颞骨中检测到病毒RNA。选择镜下有异常的切片并切成小片。从组织中提取RNA并纯化。逆转录用于cDNA合成。 DNA 在巢式 PCR 中被扩增。尽管我们无法检测到从赛璐珞包埋的胎儿颞骨中提取的风疹 RNA。 11 份样本中有 8 份检测到 α-微管蛋白 RNA (72.8%)。这些结果表明可以从档案的赛璐珞包埋的人颞骨切片中分析RNA。3)我们可以从6个标本中的1个三叉神经节标本中检测到HHV-6 DNA。这一结果可能表明HHV-6可能通过重新激活而引起一些耳科疾病。我们将不得不等待这一特定领域的进一步研究数量的增加。