Evaluation of trial denture cleansers with berberine hydrochloride

念珠菌在与义齿口炎相关的义齿菌斑中存在频率较高。清洁义齿是预防义齿口炎的重要方法。本研究研究了义齿清洁剂对白色念珠菌、热带念珠菌和光齿念珠菌的抑菌活性。试用假牙清洁剂含有盐酸小檗碱。盐酸小檗碱是一种具有较强抗真菌活性的生药成分，其本身也具有抗真菌活性。采用肉汤稀释法对洗面奶的抑菌活性进行了测定。对一种对白色念珠菌活性较强的洗面乳（S16）进行了去除丙烯酸树脂板上念珠菌、改变牙材料颜色和丙烯酸树脂板表面粗糙度的测试。这些测试表明，S16去除念珠菌的活性和一种商业假牙清洁剂一样，对物理特性没有影响。体外研究证明，一种试验义齿清洁剂具有抗真菌活性，能够去除丙烯酸树脂表面粘附的真菌细胞。因此，使用洗面奶似乎有助于预防假牙口炎。

Candida species can be found in high frequency in denture plaque related to denture stomatitis. It is important to clean dentures to prevent denture stomatitis.This study investigated the antifungal activity of trial denture cleansers against C. albicans, C. tropicalis and C. glabrata. Trial denture cleansers contain berberine hydrochloride. Berberine hydrochloride is a component of crude drugs which have strong antifungal activity and has antifungal activity itself. According to the broth dilution method, the fungicidal activity of cleansers was tested.One cleanser (S16) that showed activity against C. albicans strongly was tested for Candida removing from acrylic resin plates, color changing of dental materials and surface roughness of acrylic resin plates. These tests revealed that S16 Candida removing activity as well as one commercial denture cleanser and no effects on physical properties.In vitro studies proved that a trial denture cleanser had antifungal activity and ability to remove adhered fungal cells from acrylic resin surfaces. Hence, use of the cleanser seems to be avail in preventing denture stomatitis.