Evaluation of trial denture cleansers with berberine hydrochloride

盐酸小檗碱假牙清洁剂试验评价

• 批准号: 03670916
• 负责人: NAKAMOTO Kyoumi
• 金额: $ 1.22万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670916/
• 关键词: Denture stomatitis; Candida species; Berberine hydrochloride; Denture cleanser; 塩化ベルベリン

Candida species can be found in high frequency in denture plaque related to denture stomatitis. It is important to clean dentures to prevent denture stomatitis.This study investigated the antifungal activity of trial denture cleansers against C. albicans, C. tropicalis and C. glabrata. Trial denture cleansers contain berberine hydrochloride. Berberine hydrochloride is a component of crude drugs which have strong antifungal activity and has antifungal activity itself. According to the broth dilution method, the fungicidal activity of cleansers was tested.One cleanser (S16) that showed activity against C. albicans strongly was tested for Candida removing from acrylic resin plates, color changing of dental materials and surface roughness of acrylic resin plates. These tests revealed that S16 Candida removing activity as well as one commercial denture cleanser and no effects on physical properties.In vitro studies proved that a trial denture cleanser had antifungal activity and ability to remove adhered fungal cells from acrylic resin surfaces. Hence, use of the cleanser seems to be avail in preventing denture stomatitis.

与义齿性口炎相关的义齿菌斑中频繁发现念珠菌。清洁假牙对于预防假牙口腔炎非常重要。本研究调查了试验假牙清洁剂对白色念珠菌、热带念珠菌和光滑念珠菌的抗真菌活性。试用假牙清洁剂含有盐酸小檗碱。盐酸小檗碱是具有较强抗真菌活性的生药成分，其本身也具有抗真菌活性。采用肉汤稀释法，测试了清洁剂的杀菌活性。对一种对白色念珠菌具有较强活性的清洁剂（S16）进行了丙烯酸树脂板念珠菌去除、牙科材料变色和丙烯酸树脂板表面粗糙度的测试。这些测试表明，S16 的念珠菌去除活性与一种市售假牙清洁剂相同，并且对物理性能没有影响。体外研究证明，试验性假牙清洁剂具有抗真菌活性，并且能够去除丙烯酸树脂表面粘附的真菌细胞。因此，使用清洁剂似乎有助于预防假牙口腔炎。