Association between periodontal changes and biomechanical factors from tooth movement

牙周变化与牙齿移动引起的生物力学因素之间的关联

• 批准号: 03670973
• 负责人: TANNE Kazuo
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670973/
• 关键词: Tooth movement; Periodontal ligament (PDL); Stress; Bone remodeling; Finite element method (FEM); Immunohistochemistry; Calcitonin gene-related peptide(CGRP); calcitonin gene-related peptide(CGRP); CGRP; Substance P; 歯周組織; 骨改造

Histological changes in the periodontal ligement (PDL) from experimental tooth movements were investigated and integrated with analytical findings of stresses. A distally directed force of 250 gf was applied to the lower second premolars of adult dogs by use of a calibrated sectional arch wire with a closed loop. Mesiodistal sections of the tooth and supporting tissues were preparated at 2 hours and at 4 and 7 days after the beginning of experimental tooth movements. Further, stress levels in the PDL were analyzed by means of finite element analysis. A three-dimensional model for the analysis was constructed. The model consists of 3651 nodes and 2938 solid elements. A distally directed force of 250 gf was analytically applied to the tooth. Three principal stresses were determined in the PDL, and volumetric principal stress and shear stress component were evaluated. The nature of principal stress distributions in the PDL was highly coincident with histological findings in terms of site specific tension and compression of the PDL and alveolar bone remodeling. These results strongly emphasize the relationship between principal stresses and biological changes in the PDL, bone remodeling in particular, and hence its subsequent tooth movements.The distribution of calcitonin gene-related peptide (CGRP)- and substance P (SP)-immunoreactive (IR) nerve fibers and their correlation in the PDL of mouse incisors were examined by indirect immunofluorescence. Both CGRP-IR and SP-IR thin nerve fibers were abundant in the apical and middle third of the PDL. In the lingual protion of the incisal PDL, these nerve fibers were localized in the alveolar half of the PDL and were observed as free nerve endings. No CGRP-IR and SP-IR specialized nerve endings, such ans Ruffini-like corpuscles, were observed. In the labial PDL, CGRP-IR and SP-IR nerve fibers ran along the incisal axis. The distribution of CGRP-IR nerve fibers was very similar to that of SP-IR nerve fibers.

组织学的变化，牙周韧带（PDL）从实验性牙齿移动进行了调查，并结合分析结果的应力。通过使用具有闭合环的校准的分段弓丝，向成年狗的下第二前磨牙施加250 gf的远端定向力。在实验性牙齿移动开始后2小时、4天和7天，对牙齿和支持组织的近远中切片进行切片。此外，在PDL的应力水平进行了分析，通过有限元分析。构建了用于分析的三维模型。该模型由3651个节点和2938个实体单元组成。对牙齿施加250 gf的远端定向力。在PDL中确定了三个主应力，并评估了体积主应力和剪切应力分量。牙周膜主应力分布的性质与组织学结果在牙周膜特定部位的拉伸和压缩以及牙槽骨重建方面高度一致。这些结果强烈强调主应力和生物学变化的PDL，骨改建，特别是，因此其随后的牙齿movement.The降钙素基因相关肽（CGRP）和P物质（SP）的免疫反应（IR）神经纤维的分布和它们之间的相关性在小鼠切牙的PDL的关系进行了检查，通过间接免疫荧光。CGRP-IR和SP-IR细神经纤维在PDL的顶端和中1/3丰富。在切缘牙周膜的舌侧部分，这些神经纤维位于牙周膜的牙槽半，并观察到游离神经末梢。未观察到CGRP-IR和SP-IR特化的神经末梢，如Ruffini样小体。在唇侧PDL中，CGRP和SP免疫阳性神经纤维沿着切轴走行。CGRP免疫阳性神经纤维的分布与SP免疫阳性神经纤维的分布非常相似。

项目成果

丹根 一夫: "歯の移動に関する生体力学的研究-応力と骨改造との関連性について-" 日本矯正歯科学会雑誌. 51. 179-184 (1992)

Kazuo Tanne：“牙齿运动的生物力学研究 - 关于压力和骨重塑之间的关系 -”日本正畸学会杂志 51. 179-184 (1992)。

Kazuo Tanne.et al.: "Stress levels in the PDL and biological tooth movement." Proceedings of the International Conference on Biological Mechanisms of Tooth Movement and Craniofacial Adaptation.(1992)

Kazuo Tanne.et al.：“PDL 中的压力水平和生物牙齿移动。”

Tanne,K.,Shibaguchi,T.,Terada,Y.,Kato,J.and Sakuda,M.: "Stress Levels in the PDL and Biological Tooth Movement.in:Biological Mechanisms of Tooth Movement and Craniofacial Adaptation(Davidavitch,Z.editsy)pp.201-209" The Ohio Syaxe University, 601 (1992)

Tanne,K.、Shibaguchi,T.、Terada,Y.、Kato,J. 和 Sakuda,M.：“PDL 和生物牙齿运动中的压力水平。in：牙齿运动和颅面适应的生物机制（Davidavitch，Z）

tanne,K.,Shibaguchi´T,Terada,Y,Kato,J.and Sakuda,M.: "Stress Levels in the PDL and Biological tooch Movement,in:Biological Mechanisms of tooth Movemext and Cranofauisl Adoptation(Dosidovitch´Z.edtor)" The ohio Syaya University, 601(201-207) (1992)

tanne, K.、Shibaguchi´T、Terada, Y、Kato, J. 和 Sakuda, M.：“PDL 和生物牙齿运动中的压力水平，见：牙齿 Movemext 和 Cranofauisl 采用的生物学机制（Dosidovitch´Z.edtor） ）”俄亥俄州 Syaya 大学，601（201-207）（1992）

Kato,J.,Tanne,K.,Ichikawa,H.,Matsuo,S.,Wakisaka,S.et al.: "Distribution of Calcitonin Gone-related Peptide and Substance P-immunoreactive Nerve Fibers and their Correlation in the Periodontal Ligament of the Mouse Incisor" Acta Anatomica. 145. 101-105 (19

Kato,J.,Tanne,K.,Ichikawa,H.,Matsuo,S.,Wakisaka,S.等人：“降钙素相关肽和 P 物质免疫反应性神经纤维的分布及其在牙周膜中的相关性