Elucidation of condylar cartilage resorption mechanism in TMJ-osteoarthritis and search of a new regulatory factor

阐明颞下颌关节骨关节炎髁软骨吸收机制并寻找新的调节因子

• 批准号: 18390554
• 负责人: TANNE Kazuo
• 金额: $ 10.49万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390554/
• 关键词: mandibular condyle; SZP; Hyaluronic acid; Hyaluronidase; Mechanical stress; ヒアルロン酸; ヒアルロニダーゼ

The TMJ- osteoarthritis (OA) of the condylar cartilage absorption with movement disorder and dysfunction is one of the disease that are difficult for treatment in dental field Excessive mechanical loading to be added to the condyle as the muse is thought about with key factor of onset of OA On the other hand, it is speculated that the cartilage destruction is induced by an unknown new factor in synergy with a mechanical loading, bemuse there is the case that OA progress in a normal physiological condition. Mandibular condyle is composed of several different zone, as superficial zone from surface, cartilaginous layer, subchondral bone and bone, each layer has a different function and respond to various mechanical irritation by jaw movement Superfical zone has a lubrication function to decrease the friction, and a bone underlying in the cartilaginous layer supports the above tissues rigidly. In order to elucidate an inducing mechanism of metabolic abnormality in each layer it is importan … More t for clarifying the mechanisms of condylar cartilage destruction. We confirmed the expression of superficial zone protein which had boundary lubrication function in superficial zone of condylar cartilage in 1118 year and clarified the expression level was regulated selectively by cytokine and the magnitude of mechanical loading. In this study, we particularly focused on the downward layer (subchondral bone), and investigated the influences of subchondral bone damaged with excessive mechanical loading on the metabolisim of condylar cartilage. As a result of a 200 grams metal object was dropped to the mandibular condyle as a high magnitude impact loading, in particular, the expression of IL -1β was found in subchondral bone immunohistologically. Next, to investigate interactions between osteoblasts and chondrocytes, we used a co-culture system to culture osteoblasts monolayer together with chondrocytes in alginate beads. The mRNA expression of MMP-1, 3 and 13 in the chondrocytes by co-culture with mechanical stress-loaded osteoblasts showed increased, and the mRNA expression type II, X collagen and DNA, proteoglycan syntheses were decreased- From these results, the aggression to mandibular condyle head from the outside induce IL -1 β from subchondral bone. The results from the present study suggest that physical interactions between subchondral osteoblasts and articular chondrocytes could be responsible for the cartilage degradation in pathological changes of proceeding OA. Less

颞下颌关节骨关节炎（TMJ- osteoarthritis，OA）是一种以髁突软骨吸收为特征，伴有运动障碍和功能障碍的疾病，是牙科领域治疗的难点之一。对髁突施加过大的机械负荷被认为是OA发病的关键因素。另一方面，推测软骨破坏是由一种未知的新因素与机械负荷协同作用引起的，但也有OA在正常生理状态下进展的情况。下颌骨髁状突由表面浅层、软骨层、软骨下骨和骨组成，各层具有不同的功能，对下颌运动引起的各种机械刺激有不同的反应。为了阐明各层代谢异常的诱导机制， ...更多信息 目的：探讨髁突软骨破坏的机制。我们证实了1118年髁突软骨表浅区中具有边界润滑功能的表浅区蛋白的表达，并阐明了其表达水平受细胞因子和机械负荷大小的选择性调节。在这项研究中，我们特别关注向下层（软骨下骨），并研究过度机械负荷损伤的软骨下骨对髁突软骨代谢的影响。用200 g金属物体撞击髁突，免疫组化结果显示，IL-1 β在髁突下有表达。接下来，为了研究成骨细胞和软骨细胞之间的相互作用，我们使用共培养系统将成骨细胞单层与软骨细胞一起培养在藻酸盐珠中。与机械应力负荷成骨细胞共培养的软骨细胞MMP-1、3、13 mRNA表达增加，II、X型胶原mRNA表达减少，DNA、蛋白多糖合成减少。本研究结果提示，软骨下成骨细胞和关节软骨细胞之间的物理相互作用可能是导致OA病理变化中软骨降解的原因。少

项目成果

関節組織における潤滑・緩衝物質代謝に対する流水圧の影響

水压对关节组织润滑和缓冲代谢的影响

• 发表时间: 2007
• 作者: 柳田珠美;谷本幸太郎;田中伸明;植木雅士;北村玲子;丹根由起;神谷貴志;丹根一夫
• 通讯作者: 丹根一夫

培養ウサギ滑膜細胞と軟骨細胞のヒアルロン酸代謝に対する機械的負荷の影響

机械负荷对培养兔滑膜细胞和软骨细胞透明质酸代谢的影响

• 发表时间: 2007
• 作者: 北村玲子;谷本幸太郎;田中伸明;植木雅士;柳田珠美;丹根由起;神谷貴志;丹根一夫
• 通讯作者: 丹根一夫

Influences of chemical mediators and mechanical stress in synovial joint on lubricant metabolism

滑膜关节化学介质和机械应力对润滑剂代谢的影响

• 发表时间: 2006
• 作者: Yanagida;Y.;Honda;K.;Tanimoto;K.;Tanaka;N.;Yoneno;K.;Ueki;M.;Nakatani;Y.;Kitamura;R.;Tanne;Y.;Ohkuma;S.;Kamiya;T.;Tanne;K
• 通讯作者: K

Effects of proinflammatory cytokines on the degradation of hyaluronan in synovial cells

促炎细胞因子对滑膜细胞透明质酸降解的影响

• 发表时间: 2007
• 作者: Tanimoto;K.;Tanaka;N.;Ueki;M.;Yanagida;T.;Kitamura;R.;Tanne;Y.;Lin;YY.;Kamiya;T.;Ohkuma;S.;Huang YC.;Kunimatsu;R.;Yoshioka;M.;Tanne;K
• 通讯作者: K

Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation.

• DOI: 10.1016/j.archoralbio.2007.12.007
• 发表时间: 2008-05
• 期刊: Archives of oral biology
• 影响因子: 3
• 作者: Y. Tanne;Kotaro Tanimoto;N. Tanaka;Masashi Ueki;Yu-Yu Lin-Yu;Satoru Ohkuma;T. Kamiya;Eiji Tanaka;Kazuo Tanne