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Xyloglucan degradation systems: dissection and exploitation

木葡聚糖降解系统：剖析和利用

基本信息

批准号：
BB/I014802/1
负责人：
Gideon Davies
金额：
$ 73.01万
依托单位：
University of York
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2012
资助国家：
英国
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FI014802%2F1
关键词：
Xyloglucan degradation systems dissection exploitation

项目摘要

The complex sugars ('polysaccharides') found in the cell-walls of plants impact on all our everyday lives. They form the cotton of our clothes, the physical origins of paper and are central to all the, many, industries that derive from these products. Plant cell wall sugars are likely to form the basis of the next generation biofuel industry; one of the key governmental strategies to provide us with green, renewable and secure energy for the twenty-first century. Furthermore, the harnessing of recalcitrant sugars ('roughage') by our gut microbes is an essential part of human nutrition and intestinal transit. The gut flora are inextricably linked to human health through their metabolism of complex plant polysaccharides. The aim of this BBSRC grant application is to study how nature's catalysts, termed 'enzymes', digest and breakdown plant polysaccharides. These catalysts are the vanguard of the industries that are springing-up with the goal of harnessing the plant cell wall. Enzymes are the actors on the complex stage of the human intestine. The application focuses mainly, on one particularly difficult class of sugars; the xyloglucans. We aim to show how complex enzyme systems are required for the breakdown of this polysaccharide, how these enzymes function both at the three dimensional level and how they can be used as catalysts with potential industrial applications. We will study both key, selected representative enzymes showing useful properties and whole enzyme systems from human gut bacteria. We hope to provide the community with insight into the function and action of these catalysts and provide a foundation or their exploitation to aid in human health and technology development.

在植物细胞壁中发现的复合糖（多糖）影响着我们的日常生活。它们构成了我们衣服的棉花，纸张的物理起源，并且是所有，许多，从这些产品衍生的行业的核心。植物细胞壁糖很可能形成下一代生物燃料工业的基础;这是为我们提供世纪的绿色、可再生和安全能源的关键政府战略之一。此外，我们的肠道微生物对柠檬酸糖（“粗粮”）的利用是人类营养和肠道运输的重要组成部分。肠道植物群通过代谢复杂的植物多糖与人类健康密不可分。这个BBSRC拨款申请的目的是研究自然界的催化剂，称为“酶”，消化和分解植物多糖。这些催化剂是以利用植物细胞壁为目标的新兴产业的先锋。酶是人体肠道复杂阶段的参与者。该申请主要集中在一类特别困难的糖;木葡聚糖。我们的目标是展示如何复杂的酶系统需要这种多糖的分解，这些酶如何在三维水平上发挥作用，以及它们如何可以用作具有潜在工业应用的催化剂。我们将研究两个关键的，选择的代表性酶显示有用的特性和整个酶系统从人类肠道细菌。我们希望为社区提供对这些催化剂的功能和作用的深入了解，并提供一个基础或利用它们来帮助人类健康和技术发展。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Discovery and characterization of a new family of lytic polysaccharide monooxygenases.

DOI：
10.1038/nchembio.1417
发表时间：
2014-02
期刊：
NATURE CHEMICAL BIOLOGY
影响因子：
14.8
作者：
Hemsworth, Glyn R.;Henrissat, Bernard;Davies, Gideon J.;Walton, Paul H.
通讯作者：
Walton, Paul H.

The copper active site of CBM33 polysaccharide oxygenases.

DOI：
10.1021/ja402106e
发表时间：
2013-04-24
期刊：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
影响因子：
15
作者：
Hemsworth, Glyn R.;Taylor, Edward J.;Kim, Robbert Q.;Gregory, Rebecca C.;Lewis, Sally J.;Turkenburg, Johan P.;Parkin, Alison;Davies, Gideon J.;Walton, Paul H.
通讯作者：
Walton, Paul H.

In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions.