Study on UV-induced protease of hunam cells

为了研究人体细胞对254 nm紫外光的反应中蛋白酶活性的作用，有必要对蛋白酶进行纯化。因此，在本研究中，我们试图建立具有高水平紫外线诱导的蛋白酶活性的细胞株。由于这种诱导作用是在紫外线照射前用人干扰素预处理的RSa细胞中观察到的，而蛋白酶活性是在紫外线照射前用人干扰素预处理的细胞中观察到的，蛋白酶活性是在紫外线照射前用蛋白酶抑制剂之一antipain特异性地激活的，因此在紫外线照射后，存活细胞被克隆后立即用低剂量的抗疼痛处理的RSa细胞。紫外线照射后的存活细胞显示出高水平的蛋白酶活性，而抗疼痛药再次抑制了诱导的蛋白酶活性。除了变异细胞外，已经建立的抗干扰素细胞也表现出蛋白酶诱导作用。与亲本RSa细胞的高易变性相比，这两种变异都表现出低易变性。此外，在紫外线照射下，变异细胞的dna修复能力比亲本细胞更强。由此可见，该蛋白酶活性在紫外线照射后人体细胞的早期无差错功能中起着重要作用。

In order to study the role of protease activity in tesponse of human cells to 254 nm for-ultraviolet light it will be necessary to purify the protease. Therefore, in the present study we attempted to establish cell strains with high levels of UV-induced protease activity. As the induction was observed in RSa cells pretreated with human interferon prior to UV and the protease activity was cells pretreated with human interferon prior to UV and the protease actibity was specifically ingibited by antipain, one of proteasw inhibitors, form RSa cells treated with low deses of antipain immediately after UV asurvival cells were cloned. The survival cells showed high levels protease activity after UV iffadiation and the induced protease activity was again inhibited by antipain. Inaddition to the vatiant cells already-established interferon-resistant cells also showed protease inducibility after UV.Both the variants appeared hypomutable in contrast to hypermuability of their parent RSa cells Furthermore, more increased DNA-repir capacity was observed in the variant cells inrradiated with UV than in the parent cells.Thus, it was suggested that the protease activity play a role in error-free functions of human cells in early steps after UV.