Brain blood flow regulation of the cardiovascular center

心血管中枢的脑血流调节

• 批准号: 04044141
• 负责人: MAEDA Masanobu
• 金额: $ 0.9万
• 依托单位: University of Occupational and Environmental Health (1993)Osaka City University (1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04044141/
• 关键词: Ventrolateral Medulla; Rostral Ventrolateral Medulla; Caudal Ventrolateral Medulla; Cardiovascular Center; Cerebrovascular Circulation; Spinal Cord Circulation; Neural Pathway; Chemical Stimulation; 腹外側部; 神経性調節; ラット

In urethane-anesthetized, paralyzed and artificially ventilated rats, the neurons in the rostral ventrolateral pressor area(VLPA), origin of the sympathetic nerve activities in the brain stem, were chemically stimulated by microinjection of L-glutamate and the spinal cord blood flow(SCBF) was determined using labeled microspheres. The local SCBFs were significantly decreased and local spinal cord vascular resistances(SCVRs) were significantly increased in all spinal cord regions by unilateral chemical stimulation of the VLPA(n=12). During the chemical stimulation of the VLPA, SCBF increased in response to the changes in arterial PaCO_2, indicating that the reactivity of the spinal cord vasculature was intact(n=5). Microinjections of L-glutamate into an area adjacent to the VLPA had no effect on spinal cord circulation(n=5). These results suggest that the neurons within the VLPA may play a role in the control of spinal cord circulation.Microinjection of L-glutamate(1.7 nmol) into the ventrolateral medullary depressor area(VLDA) produced a significant(P<0.01) decrease in cerebral blood flow(CBF) and a significant(P<0.01) increase in cerebrovascular resistance(CVR) in the cerebral cortex ipsilateral to the stimulated VLDA side(n=9). Cervical sympathectomy blocked the decrease in CBF and increase in CVR elicited by chemical stimulation of the VLDA (n=10). Depression of the VLPA neurons induced by microinjection of muscimol into the VLPA blocked the CBF decrease and CVR increase responses following chemical stimulation of VLDA(n=11). These results suggest that a vasoconstrictor pathway to control cerebral vessels involves an excitatory projection from the VLDA to the VLPA and the changes in cerebral circulation are mediated by the cervical sympathetic nerves.

在麻醉、麻痹和人工通气的大鼠上，用微量注射L-谷氨酸化学刺激脑干交感神经活动的起源--头端腹外侧加压区（VLPA）的神经元，并用标记微球测定脊髓血流量（SCBF）。单侧化学刺激VLPA（n=12）可使脊髓各区域局部SCBF显著降低，局部脊髓血管阻力（SCVR）显著增加。在化学刺激VLPA时，SCBF随动脉PaCO_2的变化而增加，表明脊髓血管反应性完整（n=5）。在VLPA附近区域微量注射L-谷氨酸对脊髓循环没有影响（n=5）。结果表明，腹外侧延髓降压区（VLDA）微量注射L-谷氨酸（1.7 nmol），可使刺激VLDA侧同侧大脑皮质的脑血流量（CBF）明显减少（P<0.01），脑血管阻力（CVR）明显增加（P<0.01）（n=9）。颈交感神经切除术阻断了化学刺激VLDA引起的CBF下降和CVR增加（n=10）。在VLPA内注射蝇蕈醇（muscimol）抑制VLPA神经元，可阻断化学刺激VLDA（n=11）引起的CBF降低和CVR升高反应。这些结果表明，血管收缩通路，以控制脑血管涉及兴奋性投射从VLDA的VLPA和脑循环的变化是由颈交感神经介导的。

项目成果

Maeda M: "Regulation of cerebral circulation of the medullary cardiovascular center and its receptor." Proceeding of 2nd Gauss Symposium(Invited lecture). (in press). (1994)

Maeda M：“髓质心血管中枢及其受体的脑循环调节。”

前田正信: "脊髄循環-特に脳幹部及び末梢神経による循環調節-" 日獨医報. 39(in press). (1994)

Masanobu Maeda：“脊髓循环 - 特别是脑干和周围神经的循环调节 -”Nichidoku Iho 39（出版中）。

Maeda, M.: "Regulation of cerebral circulation of the medullary cardiovascular center and its reseptor" Proceeding of 2nd Gauss Symposium.(Invited lecture). in press. (1994)

前田，M.：“髓质心血管中枢及其受体的脑循环调节”第二届高斯研讨会论文集。（特邀报告）。

Nakai M: "Systemicand regional haemodynamic responses elicited by microinjection of N-Methyl-D-Asparatic acid into the lateral periaquductal grey matter in anaesthetized rats." Neuroscience. 58. 777-783 (1994)

Nakai M：“将 N-甲基-D-天冬氨酸显微注射到麻醉大鼠的外侧导水管周围灰质中引起全身和局部血液动力学反应。”

Maeda,M., Hayashida,Y., Nakai,M., Krieger,A.J., Sapru,H.N.: "Cerebral vasoconstructive response produced by chemical stimulation of the caudal ventrolateral medullary depressor area is mediated via the rostral ventrolateral medullary pressor area and the

Maeda,M.、Hayashida,Y.、Nakai,M.、Krieger,A.J.、Sapru,H.N.：“化学刺激尾侧腹外侧延髓降压区产生的脑血管建设反应是通过头端腹外侧延髓升压区和