Study on the function and origin of dendritic cells

树突状细胞的功能和起源研究

• 批准号: 04304031
• 负责人: IMAI Yutaka
• 金额: $ 7.04万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304031/
• 关键词: Dendritic cells; Follicular dendritic cells; Langerhans cell; Interdigitating cell; Thy-1^+ epidermal dendritic cell; Veiled cell; Function; Cellular origin; リンパ濾胞樹状細胞; 相互連結性樹状細胞; 表皮Langerhans細胞

(1)Standardization of Japanese terminology of dendritic cellsThe Japanese names of dendritic cells were standardized.(2)Follicular dendritic cell(FDC) [Cellular origin] The experiments of injection of bone marrow cells into SCID mice and the splenic implantation suggested that FDCs might be derived from the stationary cells or mesenchymal cells in lymphatic tissues, but not from bone marrow cells. [Function] The dataindicate that FDCs act as the nurse cell for B cells. The expression of IL-4 mRNA is confirmed on murine GC T cells. [Follicular dendritic cells in diseases] It is confirmed that human immuno-deficiency virus [HIV]-1-Ioaded FDCs migrate into the lymphatic circulation in the progressive stage of acquired immmunodeficlency syndrome.(3)Langerhans cell(LC)[Differentiation] The linkage of squamous metaplasia of tracheal and urinary bladder mucosal epithelium to the generation of LC is observed in vitamin A deficient rats. The generation and differentiation of LC unlike munocytes … More /macrophages are dependent on granulocyte-macrophage(GM)-CSF but not on M-CSF.[Function] The assay to evaluate the in vitro LC migration activity using Balb/cmouse earlobes was established. [Langerhans cells in diseases] The rapid diagnosis useful for immediate treatment for skin graft rejection is established. Letterer-Siwe disease is proliferative disorder because some cells are positve for proliferating cell nuclear antigen.(4)Interdigitating cell(IDC)[Cellular origin] Ligation of afferent lymphatics reveals the possibillity of direct differentiation, not via LCs, of IDCs from precursors in peripheral blood. [Differentiation] The dendritic cell differentiation factor is composed of two subcomponents. [Cell markers and characterization] Some monoclonal antibodies against stromal cells forming spienic framework are also prepared, demonstrating the plemorphism and the differentiation adapted to surrounding microenvironment of framework forming cells.(5)Thy-1^+ dendritic epidermal cell(Thy-1^+ DEC)[Generation and differentiation] Both S100beta^+ dendritic cells(LCs)and Thy-1^+ DECs participate in immune response in allogeneic akin implantation. In the early stage(2 months)of bone marrow transplantation most of epidermal T cells are CD8_<alpha> ^+ beta^-, and in the later stage CD8_<alpha> ^+ beta^+ T cells gradually increase.(6)Dendritic cells in various diseases [Pustulosis palmarls et plantaris] Morphometrical analysis of tonsillar lesions demonstrates the specific extension of secondary T-nodule and the marked increasing IDCs. [Rheumatold arthritis] A part of multinucleated giant cells in rheumatoid arthritis synovium have the immunophenotype as dendritic cell and release cytokine to promote the proliferation of synovial cells. [Idiopathic portal hypertension] In this disease IDCs markedly increase in the splenic lymphoid sheath. [Dendritic cells in tumors] Two types of dendritic cells, that is, LC-type and IDC-type dendritic cells, are found in the cancer tissues including thyroid and esophageal carcinomas. The Ia-antigen expression on cancer cells and the release of TNF-_<alpha>, GM-CSF and IL-1 from cancer cells may regulate the occurrence of dendritic cells. Less

(1)树突状细胞日语术语的标准化(2)滤泡树突状细胞(Follicular Dendritic cell，FDC)[细胞起源]SCID小鼠体内注射骨髓细胞和移植脾的实验表明，FDC可能来源于淋巴组织中的静止细胞或间充质细胞，而不是骨髓细胞。[功能]该数据表明，FDCs充当B细胞的哺育细胞。小鼠GC T细胞上存在IL-4mRNA的表达。[滤泡树突状细胞在疾病中]已证实，在获得性免疫缺陷综合征的进展期，人类免疫缺陷病毒[HIV]-1负载的FDCs迁移到淋巴循环中。(3)郎格汉斯细胞(LC)[分化]在维生素A缺乏的大鼠中，观察到气管和膀胱粘膜上皮鳞状化生与LC的发生有关。LC不同于单核细胞…的发生和分化巨噬细胞依赖粒细胞-巨噬细胞集落刺激因子(GM-CSF)，而不依赖M-CSF。[功能]建立了用Balb/小鼠耳垂评价LC体外迁移活性的方法。[朗格汉斯细胞在疾病中]快速诊断有助于皮肤移植排斥反应的即时治疗。Letterer-Siwe病是一种增殖性疾病，因为一些细胞对增殖细胞核抗原呈阳性。(4)交指细胞(IDC)[细胞起源]结扎传入淋巴管显示了从外周血中的前体细胞直接分化为IDC的可能性，而不是通过LCS。[分化]树突状细胞分化因子由两个亚成分组成。[细胞标志物及特性]本实验还制备了抗基质细胞的单抗，证实了骨架形成细胞的多形性和对周围微环境的适应能力。(5)Thy-1^+树突状表皮细胞(Thy-1^+DEC)[生成与分化]S100β+树突状细胞(LCS)和Thy-1^+DECs均参与同种异体类似物植入过程中的免疫反应。骨髓移植早期(2个月)，表皮T细胞以CD8_+T细胞为主，晚期CD8_+T细胞逐渐增多。(6)各种疾病[掌部脓疱病及足底]树突状细胞的形态计量学分析显示，继发性T结节特异性扩张，IDCs明显增多。[类风湿关节炎]类风湿关节炎滑膜中部分多核巨细胞具有树突状细胞免疫表型，并释放细胞因子促进滑膜细胞增殖。[特发性门脉高压症]在本病中，脾淋巴鞘内的IDCs明显增多。[肿瘤中的树突状细胞]在包括甲状腺和食道癌在内的癌组织中发现两种类型的树突状细胞，即LC型和IDC型树突状细胞。肿瘤细胞表面Ia抗原的表达和肿瘤细胞释放的肿瘤坏死因子α、粒细胞集落刺激因子和白介素1可能参与了树突状细胞的形成。较少

项目成果

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Mitsunori Yamakawa 等人：《现代病理学第 4 卷》淋巴结：循环障碍，9 (1994)

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Teraki Y 等人：“固定药疹中病变角质形成细胞上细胞间粘附分子的药物诱导表达。”

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Imazeki N 等人：“针对小鼠滤泡树突状细胞的新型单克隆抗体。”

Nakayama A,Hirabayashi N,Ito M,Kasai K,Fujino M,Ohbayashi M,Asai J :"White pulp reconstitution after human bone marrow transplantation." Am J Pathol. 143. 1111-1120 (1993)

Nakayama A，Hirabayashi N，Ito M，Kasai K，Fujino M，Ohbayashi M，Asai J：“人骨髓移植后的白牙髓重建。”