Immuno-morphological Study on the Function of Dendritic Reticulum Cell in Germinal Center

用光镜和电镜观察了人、小鼠和大鼠在生理、刺激、免疫和个体发生条件下淋巴结和结外淋巴滤泡中的树突状网状细胞（DRCs）。结果表明：(1)DRCs几乎局限于生发中心。将其分为无迷宫式结构和有迷宫式结构两类。前者广泛分布于整个生发中心。后者分散分布在光区的上部。(2)迷宫式结构的胞质细胞有多个复杂的胞质延伸。在横截面上，迷宫结构与抱子甘蓝相似。生发中心细胞的细胞质过程与迷宫结构无关。(3) DRCs细胞质膜呈ia样抗原阳性。在dc和淋巴样细胞之间，经常观察到桥粒和/或桥粒体连接。(4)形成淋巴结基质的成纤维网状细胞在帽区转化为典型的迷宫式结构的DRCs。(5)在发育良好的次生卵泡中，免疫复合物捕获细胞主要分布在囊帽区。(6)在淋巴结和结外淋巴滤泡生发中心的DRCs细胞表面检测到经典途径的早期补体成分及其补体受体。

The dendritic reticulum cells (DRCs) in the lymph nodes and extra-nodal lymph follicles of human, mouse and rat were observed in light and electron microscopy under the physiological, stimulated and immunized conditions as well as ontogenesis.We are presenting the results as follows:(1) The DRCs were almost confined to the germinal center. They were divided into the DRCs without labyrinth structure and with labyrinth structure. The former was widely distributed in whole germinal center. The latter was scatteredly distributed in the upper portion of the light zone.(2) The DRCs with labyrinth structures had several complicated cytoplasmic extensions. In cross sections, the labyrinth structures bore a resemblance to those of Brussels sprout. The cytoplasmic processes of germinal center cells showed no concern with the labyrinth structures.(3) The cytoplasmic plasmalemma of DRCs showed a positivity for Ia-like antigen. Between the DRCs and lymphoid cells, desmosome and/or desmosomal junctions were frequently observed.(4) The fibroblastic reticulum cells, forming the stroma of lymph nodes, were transformed into the typical DRCs with labyrinth structures in the cap area.(5) In the well-developed secondary follicles, the immune complex trapping cells, out of DRCs, were mainly localized in the cap area.(6) The early complement components in classical pathway and their complement receptors were detected on the cell surface of DRCs in the germinal centers of lymph nodes and extranodal lymph follicles.