Rapid and Sensitive Microanalyzes of ^<13>C and ^<15>N tracers.

1. 成功建立气相色谱/燃烧/同位素比质谱仪（GC/C/IRMS）。利用该系统建立了一种快速简便的分析方法，用于测定湿地收集的气泡甲烷碳同位素比值、湖泊水样中溶解无机碳（SIGMA CO_2），以及水稻根际^<13>C- ch_4产量的示踪剂研究。此外，还开展了干木白蚁（Neotermes koshuenies Shiraki）对氮的分子固定和浮游植物的初级产量的研究。3 .建立了制备生物材料的高真空生产线，并对biwa、suwa、teganuma和白蚁生态系统中不同种类的生物样品进行了碳氮同位素比值测定。采用不同种类的浮游生物网和手工浸泡，成功地进行了水生微生物的粒度分馏。

1. Gas chromatography/Combusion/Isotope Ratio Mass Spectrometer (GC/C/IRMS) was successfully established. The system was employed to develop a rapid and simple analytical methods for measuring carbon isotope ratio of bubble methane collected from wetlands, dissolved inorganic carbon (SIGMA CO_2) in lake water samples, and ^<13>C tracer studies of ^<13>C-CH_4 production from a rhizosphere of rice plants.2. ^<15>N_2 and ^<13>C tracer studies were also developed for assessing the molecular dinitrogen fixation by the dry-wod termite, Neotermes koshuenies Shiraki, and the primary production by phytoplankton in Lake Biwa.3. High vacuum line for the prepation of biological meterials were built and carbon and nitrogen isotope ratios were measured for various kinds of biogenic samples collected from L.Biwa, L.Suwa, L.Teganuma and a termite ecosystem.4. Size fractionation of aquatic microorganisms was successfully performed by using a various kind of plankton net and soating by hand.