Isotope Biogeochemical Structure of Biosphere-New Paradigm

生物圈同位素生物地球化学结构-新范式

• 批准号: 05403003
• 负责人: WADA Eitaro
• 金额: $ 23.68万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05403003/
• 关键词: stable isotope ratio; carbon; nitrogen; Lake Biwa; Lake Baikal; forest; termite; GC; C; IRMS; バイカル湖; 熱帯林; 高等植物; シロアリ; 安定同位体; 色素; 炭素・窒素安定同位体比; 脱窒; 食物連鎖; 炭酸ガスの増加

A system for the measurement of carbon and nitrogen stable isotope ratios has been established in Center for Ecological Research, Kyoto University, which is one of the central and leading place in the field of Isotope Ecology in the world.On line measurement system to determine methane delta ^<13>C and total inorganic carbon delta ^<13>C in fresh waters has been developed by using a gas chromatograph/Combustion/Isotope Ratio Mass Spectrometer (GC/C/IRMS) .A delta <@D115@>D1N (NO<@D23@>D2<@D1-@>D1) -<@D7[NO<@D23@>D2](/)Cl@>D7 isotopic map was successfully conpleted for assessing the dynamics of nitrogen in a forested ecosystem. A delta <@D113@>D1C (CH<@D24@>D2) -delta D (CH<@D24@>D2) map was also made for assessing the production mechanisms of methane in various kinds of wetland involving paddy fields.Isotope biogeochemical structures of a food web in Lake Biwa and Lake Baikal was obtained. Importance of N_2 fixation in a termite-protozoa-N_2 fixing bacteria association was clarified under in-situ conditions.In forested ecosystems, a relationshi between light inlensity and a vertical trend of leaf of delta ^<13> Cwas established.

京都大学生态研究中心建立了碳氮稳定同位素比值测定系统，并<13><13>利用气相色谱/燃烧/同位素比值质谱联用仪（GC/Combustion/Isotope Ratio Mass Spectrometer），建立了沃茨中甲烷δ C和总无机碳δ C的在线测定系统（GC/C/IRMS），成功地绘制了δ &lt;@D115@&gt; D1 N（NO&lt;@D23@&gt;D2&lt;@D1-@&gt;D1）-&lt;@D7[NO&lt;@D23@&gt;D2]（/）Cl@&gt; D 7同位素图，并用于评价森林生态系统中氮的动态。还绘制了δ &lt;@D113@&gt; D_1 C（CH&lt;@D24@&gt; D_2）-δ D（CH&lt;@D24 @&gt; D_2）图，以评价不同类型稻田湿地甲烷的产生机制，并获得了琵琶湖和贝加尔湖食物网的同位素地球化学结构。在原位条件下阐明了白蚁-原生动物-固氮菌联合体固氮的重要性，并建立了森林生态系统中光照强度与三角洲叶片垂直走向之间的关系<13>。

项目成果

Miyajima, T., Yamada, Y., Hanba, T. Y.,Yoshii, K., Koitabashi, T. and Wada, E.: "Detemining the stable isotope ratio of total dissolved inorganic carbon in lake water by GC/C/IRMS." Limnol. Oceanogr.40(5). 994-1000 (1995)

Miyajima, T.、Yamada, Y.、Hanba, T. Y.、Yoshii, K.、Koitabashi, T. 和 Wada, E.：“通过 GC/C/IRMS 测定湖水中总溶解无机碳的稳定同位素比率。

Yoshioka,T.,Wada,E.and Hayashi,H.: "A stable isotope study on seasonal foodweb dynamics in a eutrophic lake." Ecology. 75. 835-846 (1994)

Yoshioka,T.、Wada,E. 和 Hayashi,H.：“富营养化湖泊季节性食物网动态的稳定同位素研究。”

和田英太郎: "安定同位体は何を語るか" 遺伝. 47. 10-14 (1993)

Eitaro Wada：“稳定同位素告诉我们什么？” 47. 10-14 (1993)

Ichikawa, T., Hasegawa, K., Shimizu, I., Kataoka, H.and Suzuki, A.: "Structure of neurosecretory cells with immunoreactive diapause hormone and pheromone biosynthesis activating neuropeptide in the silkworm." Bombxy mori.Zool.Sciences. 12. 703-712 (1995)

Ichikawa, T.、Hasekawa, K.、Shimizu, I.、Kataoka, H. 和 Suzuki, A.：“蚕体内具有免疫反应性滞育激素和信息素生物合成激活神经肽的神经分泌细胞结构。”

Matsuura Y.and Wada,E.: "Carbon and nitrogen stable isotope ratios in marine organic matters of the coastal ecosystem in Ubatuba,southem Brazil." J.Brazilian Asso.for Advancement of Sci.46. 141-146 (1994)

Matsuura Y. 和 Wada,E.：“巴西南部乌巴图巴沿海生态系统海洋有机物中的碳和氮稳定同位素比率。”