Development of Active Modulation Image Processing Ultra High Resolution Electron Microscope

研究工作已按计划进行。主要研究成果如下：(1)研制成功了加速型电压调制型主动调制图像处理电子显微镜。我们驱动高压电源的反馈电路，将调制电压叠加到加速电压上。这使得能够通过高速实现定制的散焦调制。(2)我们发展了一种新的散焦调制技术，称为功能化照射时间控制法，该方法是根据散焦调制图像处理中的权函数来控制初级光束的照射时间。(3)将这种功能化的辐照时间控制方法应用于主动图像处理电子显微镜，成功地实现了金粒子的无像差观测。这一观测证实了Au原子的位置可以被准确地确定。(4)将该方法应用于生物样品的正聚焦条件下的电子显微镜观察。值得注意的是，这些生物样本不再被染色以进行电子显微镜观察。这显著减少了染色引起的伪影。

Research has been proceeded as planned. The results are summarized as follows ;(1) Accelerating voltage modulation type active modulation image processing electron microscope has been successfully developed. We drove the feed back circuit of high voltage power supply in order to superimpose the modulation voltage onto accelerating voltage. This enabled tailored defocus-modulation to be attained by high speed. The result has been reported in paper (1).(2) We have developed a novel defocus-modulation technique, called functionized irradiation time control method, This method is to control irradiation time of primary beam according to the weight function for defocus-modulation image processing. The first data were described in the paper (2).(3) Applying this functionized irradiation time control method for active image processing electron microscopy we succeeded in the aberration-free observation of Au-particle. This observation has confirmed that the position of Au-atoms can be determined accurately. (paper (3)).(4) We have been involved in application of this method for electron microscopic observation of biological samples under just-focus condition. It is noted that these biological samples are no more stained for electron microscopic observation. This reduces significantly the artifact caused by the staining.