Real-Time Active Modulation Electron Microscopy

本课题研究成果如下：(1)成功构建了用于实时有源调制处理的加速电压调制系统。(2)利用电子光学系统的特性导出调制信号，通过光纤将其转换到安装在高压罐中的调制系统中。(3)通过将校正信号叠加到调制信号上，成功地补偿了由加速电压电源反馈系统响应引起的调制信号变形。(4)在实时有源调制电子显微镜下观察未染色的生物样品，目前已观察到高对比度图像。然而，整个电子显微镜的稳定性逐渐恶化，这限制了高分辨率的观察。

The results obtained through the present research project are as follows :(1) Accelerating voltage modulation system for real-time active modulation processing has been successfully constructed.(2) Modulation signals which has been derived from the characteristics of electron optical system was transformed through optical fiber to the modulation system installed in high voltage tank.(3) The deformation of modulation signals due to the response of feedback system of accelerating voltage power supply was successfully compensated by overlapping the correction signals onto the modulation signals.(4) We have been involved in observing unstained biological samples under the real-time active modulation electron microscope and high contrast image have been observed so far.However, the stability of the entire electron microscope has been gradually deteriorating and this limited the high-resolution observation.