Atopy ; genetic epidemiology in Japanese population

特应性；

• 批准号: 04557029
• 负责人: MORIMOTO Kanehisa
• 金额: $ 11.65万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557029/
• 关键词: atopy; IgE antibody; IgE receptor; polymorphysm; genetic epidemiology; molecular genetics; lifestyle; health promotion and prevention of disease; アレルギー; IgEレセプター; 遺伝的多型; 連鎖解析; PCR; 喫煙; イムノグロブリンE; 超合金; スクリーニング

We confirmed the association between atopy and chromosome llq in collabolation with University of Oxford research group. In order to investigate this finding in Japanese population, we recruited 500 random patients and control invididuals and DNA were extracted by usual method. There was a significant association between atopy and an allelic polymorphysm found in Fc epsilon RI beta gene. However, no association was found between atopy and 5' and 3' flanking genes, TCN1 and CD20 on chromosome llq. These results confirm that there is a candidate gene for atopy mapping around Fc epsilon RI beta gene.In English population, a variant, Leu 181 in Fc epsilon RI beta showed a storong association with atopy. As an easy tool to detect this variant, ARMS method was developed using PCR technique. Also succeeded in detecting very low number copies of DNA from small blood samples (100 mu) and urine. This might help to collect blood samples from Africa, Asia and South America countries where it takes a lot of time to move under high temperature.We therefore collected about 1000 DNA samples in western Australia and east Africa. Leu 181 variant was detected in those samples and is significantly associated with atopy. A new variants were found to be associated with allergic rhinitis in Japanese population. New ARMS tests were under way to investigate in our population.Recently, certain number of candidate gene for atopy have been documented in the world. ARMS tests are also available to test those candidates. This suggests that we are able to find high risk individuals for atopy. In combination with quantitating environmental factors, we can predict the development of atopy during pregnancy.

我们与牛津大学研究小组合作证实了特应性与染色体llq之间的关联。为了在日本人群中调查这一发现，我们随机招募了500名患者，对照组用常规方法提取DNA。在Fc ε RI β基因中发现的等位基因多态性与特应性之间存在显著关联。然而，特应性与染色体11q上的5'和3'侧翼基因TCN 1和CD 20之间没有发现关联。这些结果证实了Fc β RI β基因周围存在一个特异性定位的候选基因，在英国人群中，Fc β RI β的一个变异体Leu 181与特异性有很强的关联。作为一种简单的工具来检测该变异，ARMS方法是利用PCR技术开发的。还成功地从少量血液样本（100 μ）和尿液中检测出非常低数量的DNA拷贝。这可能有助于从非洲、亚洲和南美洲国家收集血液样本，因为这些国家在高温下移动需要很长时间。因此，我们在澳大利亚西部和东非收集了大约1000个DNA样本。在这些样本中检测到Leu 181变异体，并且与特应性显著相关。在日本人群中发现了一种与过敏性鼻炎相关的新变体。新的ARMS检测方法正在我国人群中进行研究，近年来，国际上已发现了一定数量的特应性遗传病候选基因。ARMS测试也可用于测试这些候选人。这表明，我们能够找到高风险的个人过敏症。结合环境因素的量化，可以预测孕期特应性的发生。

项目成果

TARO SHIRAKAWA: "Association between atopy and variants of the β subumit of the high-attinity immunogiobulin E receptor" Nature Geuetics. 7. 125-130 (1994)

TARO SHIRAKAWA：“特应性与高亲和性免疫球蛋白 E 受体 β 亚基变体之间的关联”Nature Geuetics 7. 125-130 (1994)。

森本 兼曩,白川 太郎,日下 克則,近藤 弘: "ライフスタイルと免疫学的防御機構" ライフサイエンス出版株式会社, 7 (1992)

森本兼弘、白川太郎、草坂克典、近藤博：《生活方式与免疫防御机制》生命科学出版社，7（1992）

Takeuchi,T.: "Spin trapping of superoxide released by opsonized asbestos from human promyelocytic leukemia cell line,HL60." Biochem.Biophys.Res.Com.194. 57-64 (1993)

Takeuchi,T.：“人类早幼粒细胞白血病细胞系 HL60 中调理石棉释放的超氧化物的自旋捕获。”

Morimoto,K.: "Chromosome alterations in peripheral lymphocytes as indices of lifestyle and genotoxicity." Int.Arch.Occup.Env.Health. 65. 37-41 (1993)

Morimoto,K.：“外周淋巴细胞的染色体改变作为生活方式和基因毒性的指标。”

Sandford, A.J., Shirakawa, T., Moffatt, M.F., Daniels, S.E., Ra, C., Faux, J.A., Young, R.P., Nakamura, Y., Lathrop, G.M., Cookson, W.O.C.M., and Hopkin, J.M.: "Localisation of atopy and beta subuit of high-affinity IgE receptor (Fc epsilonRI) on chromoso

Sandford, A.J.、Shirakawa, T.、Moffatt, M.F.、Daniels, S.E.、Ra, C.、Faux, J.A.、Young, R.P.、Nakamura, Y.、Lathrop, G.M.、Cookson, W.O.C.M. 和 Hopkin, J.M.：“本地化