BIOLOGICAL RECONSTRUCTION OF PERIODONTAL TISSUE USING CYTOKINE AND FIBROUS COLLAGEN MEMBRANE

骨形态发生蛋白（BMP）是一种生长和分化因子，当它与不溶性骨基质（IBM）作为载体植入时，刺激未成熟的间充质细胞产生类似于软骨内骨化的过程。该运营商的角色仍然未知，IBM以外的运营商尚未被调查。我们设计了一种纤维胶原膜（FCM），目的是将其与骨形态发生蛋白（BMP）联合收割机结合，并植入皮下进行异位成骨。在本研究项目中，我们首先成功地在FCM/BMP植入物中独立地产生了骨和软骨的异位形成。因此，为了阐明BMP的载体依赖性表型表达现象背后的机制，我们制备并装置了至少六种不同类型的载体，将它们与色谱纯化的BMP组合，并比较植入后的表型表达。除IBM和FCM外，新的BMP载体包括多孔羟基磷灰石颗粒（PPHAP）、固体羟基磷灰石颗粒（SPHAP）、蜂窝状羟基磷灰石和纤维玻璃膜（细和粗FGM）。最引人注目的发现是，粗FGM/BMP仅在膜内诱导软骨，而PPHAP/BMP仅在PPHAP孔内诱导骨，发现SPHAP/BMP和细FGM/BMP根本不诱导骨或软骨。蜂窝状HAP由圆柱形块（1.4 x 0.7 mm）和7个直径为0.12 mm的管状孔组成，诱导了非常独特的骨形状，在每个管状孔的中心产生柱状骨，而不是在管状孔的内表面上。PPHAP中的孔隙为血管提供了空间，这对于直接成骨是可行的，而FGM拒绝血管，但允许间充质细胞进入膜，这为软骨形成提供了可行的环境。上述结果表明，几何因素在FCM的使用中是重要的，

Bone morphogenetic protein (BMP) is a growth and differntiation factor that stimulates immature mesemchymal cells to create a process similar to endochonral ossification when it is implanted with insoluble bone matrix (IBM) as a carrier. Roles of this carrier is still unkown and carriers other than IBM have not been investigated. We have deviced a fibrous collagen membrane (FCM), in order to combine it with BMP and to implant subcutanuously for ectopic bone formation. In this research project, first we succeeded in generating the ectopic formation of bone and cartilage independently in FCM/BMP implant. Thus, in order to clarify the mechanism behind the phenomenon of carrier-dependent pehonotype expression by BMP, we prepared and deviced at least six different types of carriers, combined them with the chromatographically-purified BMP, and compared the phenotype expression upon implantation. New BMP-carriers other than IBM and FCM included the porous particles of hydroxyapatite (PPHAP), solid particles of hydroxyapatite (SPHAP), honeycomb shaped hydroxyapatite and fibrous glass membrane (fine and coarse FGM). Most striking finding was that the coarse FGM/BMP induced only cartilage inside the membrane, while the PPHAP/BMP induced only bone within the pore of the PPHAP.It was found that SPHAP/BMP and the fine FGM/BMP did not induce bone nor cartilage at all. Honeycomb HAP, which is consistedof cylindrical blocks (1.4 x 0.7 mm) with seven tubular holes of 0.12 mmdiameter, induced very unique shape of bone, generating the columnar bone in the center of each tubular hole, not on the inner surface of tubular holes. It was interpreted that pores in the PPHAP provided the space for vasculature which was feasible for direct bone formation, while FGM rejected vasculature but permitted mesenchymal cells entering into the membrane, which provided a feasible circumstances for cartilage formation. Above results indicate that geometrical factors are important in the use of FCM and