Tissue engineered periodontal regenerations on replanted tooth and artificial tooth

再植牙和人造牙的组织工程牙周再生

• 批准号: 11470385
• 负责人: KUBOKI Yoshinori
• 金额: $ 8.13万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470385/
• 关键词: periodontal regeneration; cementum regeneration; BMP; FGF; tissue engineering; titanium mesh; hydroxyapatite coating; laser perforated membrane

The aims of this study were to extend the regeneration of periodontal tissues in the denuded roots of replanted teeth and in the artificial roots of teeth. Recombinant human BMP-2 was combined with fibrous collagen membrane(FCM)as reported(FCM/BMP). As a replanting system, premolars of beagle dogs were carefully extracted and treated. Before replantation, their periodontal ligaments(PL)and cementum were partially removed and were covered with FCM/BMP.Although the characteristic perpendicular collagen fibers were histologially observed between the newly formed cementum and alveolar bone, extent of regeneration was limited, often accompanied by ankyosis and resorption of roots. Then we devised a artificial root system, in which a titanium rod was surrounded by titanium-fiber mesh(TM-TR). This device(TM-TR)effectively induced layered bone(cementum-like tissue)within the Ti-mesh, when combined with rhBMP-2 and implanted into rat skin. Two other BMP-carriers, honeycomb hydroxyapatite and CPSA bioglass fibers were developed. In order to prevent ankylosis, we also devised laser-perforated collagen membrane. Together with new artificial teeth and new BMP carrier devices, dental implant with periodontal ligaments became ready-to-test stage of development.

本研究的目的是延长断根再植牙和人工牙根中牙周组织的再生。将重组人BMP-2与已报道的纤维胶原膜（FCM）复合（FCM/BMP）。作为一个再植系统，beagle犬的前磨牙被仔细提取和处理。在再植前，部分切除牙周韧带（PL）和牙骨质，用FCM/BMP复合物覆盖，在牙骨质和牙槽骨之间形成垂直的胶原纤维，但再生程度有限，常伴有牙根的强直和吸收。然后，我们设计了一种人工牙根系统，其中钛棒被钛纤维网（TM-TR）包围。当与rhBMP-2结合并植入大鼠皮肤时，该器械（TM-TR）可有效诱导钛网内的分层骨（骨水泥样组织）。开发了另外两种BMP载体，蜂窝状羟基磷灰石和CPSA玻璃纤维。为了防止关节强直，我们还设计了激光穿孔胶原膜。随着新型人工牙和新型BMP载体装置的出现，带牙周韧带的牙种植体进入了准备测试的发展阶段。

项目成果

Jin QM: "Effects of Geometry of hydroxyapatite as a cell substratum in BMP-induced ectopic bone formation."J Biomed Mat Res. 51. 491-499 (2000)

Jin QM：“羟基磷灰石作为细胞基质的几何形状对 BMP 诱导异位骨形成的影响。”J Biomed Mat Res。

Jin QM, Takita H, Kohgo T, Atsumi K, Itoh H and Kuboki Y: "Effects of Geometry of hydroxyapatite as a cell substratum in BMP-induced ectopic bone formation."J Biomed Mat Res. 51. 491-499 (2000)

Jin QM、Takita H、Kohgo T、Atsumi K、Itoh H 和 Kuboki Y：“羟基磷灰石作为细胞基质的几何形状对 BMP 诱导异位骨形成的影响。”J Biomed Mat Res。

Kikuchi M.: "Laser perforated collagen membrane : pore size-dependent bone induction as a new BMP carrier."J.Hard.Tissue Biology. 9. 79-89 (2000)

Kikuchi M.：“激光穿孔胶原膜：作为新的 BMP 载体的孔径依赖性骨诱导。”J.Hard.Tissue Biology。

Ono I, Tateshita T and Kuboki Y: "Promotion of the physical strength of hydroxyapatite implants by prostaglandin E1 and recombinant human bone morphogenetic protein."j Biomed Mater Res. (In press).

Ono I、Tateshita T 和 Kuboki Y：“通过前列腺素 E1 和重组人骨形态发生蛋白促进羟基磷灰石植入物的物理强度。”j Biomed Mater Res。

Jin QM, Kuboki Y: "Effects of Geometry of Hydroxyapatite as a cell substratum in BMP- induced ectopic bone formation."J.Biomed Mat Res. 51. 491-499 (2000)

Jin QM，Kuboki Y：“羟基磷灰石的几何形状作为细胞基质对 BMP 诱导的异位骨形成的影响。”J.Biomed Mat Res。