Tissue engineered periodontal regenerations on replanted tooth and artificial tooth

本研究的目的是延长断根再植牙和人工牙根中牙周组织的再生。将重组人BMP-2与已报道的纤维胶原膜（FCM）复合（FCM/BMP）。作为一个再植系统，beagle犬的前磨牙被仔细提取和处理。在再植前，部分切除牙周韧带（PL）和牙骨质，用FCM/BMP复合物覆盖，在牙骨质和牙槽骨之间形成垂直的胶原纤维，但再生程度有限，常伴有牙根的强直和吸收。然后，我们设计了一种人工牙根系统，其中钛棒被钛纤维网（TM-TR）包围。当与rhBMP-2结合并植入大鼠皮肤时，该器械（TM-TR）可有效诱导钛网内的分层骨（骨水泥样组织）。开发了另外两种BMP载体，蜂窝状羟基磷灰石和CPSA玻璃纤维。为了防止关节强直，我们还设计了激光穿孔胶原膜。随着新型人工牙和新型BMP载体装置的出现，带牙周韧带的牙种植体进入了准备测试的发展阶段。

The aims of this study were to extend the regeneration of periodontal tissues in the denuded roots of replanted teeth and in the artificial roots of teeth. Recombinant human BMP-2 was combined with fibrous collagen membrane(FCM)as reported(FCM/BMP). As a replanting system, premolars of beagle dogs were carefully extracted and treated. Before replantation, their periodontal ligaments(PL)and cementum were partially removed and were covered with FCM/BMP.Although the characteristic perpendicular collagen fibers were histologially observed between the newly formed cementum and alveolar bone, extent of regeneration was limited, often accompanied by ankyosis and resorption of roots. Then we devised a artificial root system, in which a titanium rod was surrounded by titanium-fiber mesh(TM-TR). This device(TM-TR)effectively induced layered bone(cementum-like tissue)within the Ti-mesh, when combined with rhBMP-2 and implanted into rat skin. Two other BMP-carriers, honeycomb hydroxyapatite and CPSA bioglass fibers were developed. In order to prevent ankylosis, we also devised laser-perforated collagen membrane. Together with new artificial teeth and new BMP carrier devices, dental implant with periodontal ligaments became ready-to-test stage of development.