Role of geometrical factors of cell-substratum in BMP-induced osteogensis, topology of bone formation

细胞基质几何因素在 BMP 诱导的成骨、骨形成拓扑中的作用

• 批准号: 07457437
• 负责人: KUBOKI Yoshinori
• 金额: $ 4.8万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457437/
• 关键词: BMP; Carrier; Porous hydroxyapatite; Porosity; Pore sizes; geometry; Reconstruction; 骨形成; トポバイオロジー

The effect of matrix geometry upon the osteogenis induced by bone morphogenetic protein (BMP) was studied. A series of five porous hydroxyapatites with different pore sizes, 106-212,212-300,300-400,400-500, and 500-600mum, was prepared. A block (approximately 5x5x1mm, 40.0mg) of each hydroxyapatite ceramics was combined with 4mug of recombinant human BMP-2 and implanted subcutaneously into the back of rat. Osteoinductive ability of each implant was estimated by quantifying osteocalcin content (OC) and alkaline phosphatase activity (ALP) in the implant up to 4 weeks after implantation. Comparison of the ALP at 2 week and the OC at 4 weeks after implantation revealed that the highest amount of bone was produced in the implants with pore size of 300-400mum. In the ceramcs with smaller of larger pore sizes, the amount of formation decreased as the pore size deviated from 300-400mum. The results indicated that the optimal pore size of attachment, differentiation and growth of osteoblasts and vascularization is approximately 300-400mum. This study using chemical identical but geometrically different cell substrata is the first demonstration that a matrix with a certain geometrical size is most favorable for cell differentiation.

研究了基质几何形状对骨形态发生蛋白（BMP）诱导成骨的影响。制备了一系列不同孔径（106-212、212-300、300-400、400-500、500-600μm）的五种多孔羟基磷灰石。每个羟基磷灰石陶瓷块（约5x5x1mm，40.0mg）与4杯重组人BMP-2组合并皮下植入大鼠背部。通过在植入后 4 周内量化植入物中的骨钙素含量 (OC) 和碱性磷酸酶活性 (ALP) 来评估每个植入物的骨诱导能力。植入后第2周的ALP和第4周的OC的比较显示，孔径为300-400μm的种植体产生的骨量最高。在孔径较小或较大的陶瓷中，当孔径偏离300-400μm时，生成量减少。结果表明，成骨细胞附着、分化、生长和血管形成的最佳孔径约为300-400μm。这项研究使用化学成分相同但几何形状不同的细胞基质，首次证明具有一定几何尺寸的基质最有利于细胞分化。

项目成果

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