Reconstruction of periodontal tissues by BMP combined with functional fibrous collagen membrane

BMP联合功能性纤维胶原膜重建牙周组织

• 批准号: 06557095
• 负责人: KUBOKI Yoshinori
• 金额: $ 1.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557095/
• 关键词: BMP; Carrier; Fibrous collagen membrane; Periodontium; Cementum; Periodontal membrane; Reconstruction

We have previously establish a system of ectopic chondrogenesis and osteogenesis by using bone morphogenetic proteins (BMPs) and various kind of carriers. We found that BMP-induced cell-differentiation is highly dependent on the nature of these carriers. These BMP-carriers were classified into three types : fiber-, particle-and block-types. (1) The fiber-type includes fibrous collagen membrane (FCM) and fibrous glass membrane (FGM). (2) The particle-type : collagen beads, porous particles of hydroxyapatite (PPHAP) and solid particles of hydroxyapatite, and (3) the block-type : porous blocks of hydroxyapatite and honeycomb-shaped hydroxyapatite. By FCM carrier, we could regenerate not only alveolar bone but also cementum and periodontal membrane in cat. Furthermore, we found a strong synergistic effect between rhBMP-2 and S300 BMP.Half of the minimum effective amount of the BMPs cocktail (30 ug) which was combined with 10 mg of collagen beads as a carrier did not induce detectable bone formation. Also, 1.2 ug of rhBMP-2 (gifted from Yamanouchi Co.) did not induce detectable bone. However, 20 ug of BMPs cocktail, 1.2 ug of rhBMP2 and 10 mg of collagen beads were combined, prominent bone formation (2 IU of ALP and 3.5 mg of calcium per implant) were observed at 2 weeks after implantation. The results indicated that certain component (s) in the BMPs cocktail possessed a potent synergistic effect with rhBMP-2. Above results will open a new avenue of clinical application of BMP for periodontal regeneration.

我们以前已经建立了利用骨形态发生蛋白(BMPs)和各种载体进行异位软骨形成和成骨的系统。我们发现BMP诱导的细胞分化高度依赖于这些载体的性质。骨形态发生蛋白载体可分为纤维型、颗粒型和块状型。(1)纤维类型包括纤维胶原膜(FCM)和纤维玻璃膜(FGM)。(2)颗粒型：胶原珠、羟基磷灰石多孔颗粒(PPHAP)和羟基磷灰石固体颗粒；(3)块状：羟基磷灰石多孔块和蜂窝状羟基磷灰石。FCM载体不仅可以再生牙槽骨，还可以再生牙骨质和牙周膜。此外，我们还发现，重组人骨形态发生蛋白-2和S300骨形态发生蛋白之间有很强的协同作用。骨形态发生蛋白鸡尾酒的最低有效剂量(30ug)的一半与10 mg的胶原珠作为载体不能诱导可检测到的骨形成。此外，1.2微克重组人骨形态发生蛋白-2(来自Yamanouchi Co.)没有诱导出可检测到的骨骼。20ug BMPs鸡尾酒+1.2ug重组人BMP2+10mg胶原蛋白微珠，种植后2周有明显的骨形成(2IU/颗碱性磷酸酶/颗种植体，3.5 mg/颗钙)。结果表明，骨形态发生蛋白鸡尾酒中的某组分(S)与重组人骨形态发生蛋白-2具有较强的协同作用。以上结果将为BMP在牙周再生中的临床应用开辟一条新的途径。

项目成果

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