Stem cell culture system with optimal spaces and anti-gravitational stimuli

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We report here a new invention of an anti-gravity device for cell culture, in that we can invert the position of conventional culture dishes up-side-down in the culture medium. Also, we can rotate the 3D scaffolds of any kind in culture medium by this device. Our hypothesis is: if we change the direction of gravitational force on cell systematically, we can eventually detect the effects of gravity on the behaviors of cells. In this paper we described the details of the structures and concept of anti-gravitational device, and also some of the preliminary results of application of the device for osteoblasts MC-3T3 E1 and cancer cells MG 63. Results included remarkable morphological changes of the cells under the anti-gravitational environment, with 10-20 times higher RNA values of actin and collagen, indicating total rearrangement of cyto-skeletons and protein productions.