The establishment of the standard method for rat embryo-cryopreservation method and for the network.

建立大鼠胚胎冷冻保存方法和网络的标准方法。

• 批准号: 04558012
• 负责人: KASAI Noriyuki
• 金额: $ 3.46万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04558012/
• 关键词: Rat; Obtaining embryos; Embryo cryopreservation; Vitrification; Cryoprotectant; Net work; 2Cell embryo; Slow freezing method; ラット受精卵凍結の慢法; ラット受精卵凍結ガラス化法; ラット受精卵採取方法; 誘起排卵法; 自然交配法; 凍結保護液; 凍結保存受精卵データベース; 凍結受精卵情報ネットワーク

Vitrification method for rat embryo cryopresearvation has not been established yet. In addition, it is not clear which facilitles are cryopresearving the rat embryos. The purpose of this research is as follows ;1. The establishment of the standard method for rat embryo-cryopresear vation.2. The development of the vitrification method for rat embryos.3. The establishment of the effective method for obtaining rat embryos.4. The establishment of the database and the network system for rat embryo-cryopresearvation in Japan.The best method for rat embryo-cryopresearvation is the method that Mizuno et al. developed. They reported and we also think that the 2 cell embryos are the best stage for cryopresearvation.Hirabayashi et al. and Kasai et al. improved the vitrification methods previously reported. But the methods have not been completed.Ishikawa et al. and Yamazaki et al. improved the methods for obtaining rat embryo-cryopresearvation by the induced ovulation with PMSG and hCG and these methods are useful for practice.The database and the network for rat embryo-cryopresearvation have not been established yet in this study. We will continue to do these purpose.

大鼠胚胎玻璃化冷冻方法尚未建立。此外，尚不清楚哪些设施正在冷冻研究大鼠胚胎。本研究的目的如下：1.大鼠胚胎冷冻标准方法的建立.大鼠胚胎玻璃化冷冻方法的建立.建立有效的大鼠胚胎获取方法.建立了日本大鼠胚胎冷冻研究数据库和网络系统，认为Mizuno等开发的方法是大鼠胚胎冷冻研究的最佳方法。Hirabayashi等和加塞等对已有的玻璃化冷冻方法进行了改进。但目前的方法还不完善，石川等和山崎等改进了PMSG和hCG诱导排卵的大鼠胚胎冷冻方法，具有实用价值，本研究尚未建立大鼠胚胎冷冻研究的数据库和网络。我们将继续实现这些目标。

项目成果

Kasai,M.Hiraishi,M.Nureki,I.Zhu,S.E.Sakurai,T.Machida,T.: "Influence of the mucin coat on the survival of rabbit and mouse embryos stored at 0℃." J.Exp.Zool.267. 344-349 (1993)

Kasai、M. Hiraishi、M. Nureki、I. Zhu、S. E. Sakurai、T. Machida、T.：“粘蛋白外壳对 0℃ 保存的兔子和小鼠胚胎存活的影响。” 267. 344-349 (1993)

Kasai,M.,Nishimori,M.,Zhu,S.E.,Sakurai,T.Machida,T.: "Survival of mouse morulae vitrified in an ethylene Glycol based solution after eysosure to the solution at various temperatures." Biol.Reprod.47. 1134-1139 (1992)

Kasai，M.，Nishimori，M.，Zhu，S.E.，Sakurai，T.Machida，T.：“在不同温度下对溶液进行 eysosure 后，小鼠桑葚在乙二醇基溶液中玻璃化的存活。”

Zhu, S.E., Kasai, M., Otoge, H., Sakurai, T., and Machida, T.: "Cryopreservation of expanded mouse blastocysts by vitrification in ethylene glycol-based solutions." J.Reprod.Fertil.98. 139-145 (1993)

Zhu, S.E.、Kasai, M.、Otoge, H.、Sakurai, T. 和 Machida, T.：“通过在乙二醇溶液中玻璃化冷冻保存扩增的小鼠囊胚。”

Miyoshi,I.,Yamashita,T.,Aoyama,S.,Sasaki,N.,Maki,K.,Kasai,N: "Effect of environmental factors on the yield of mouse embryos obtained after hormone induced superovalation" Lab.Anim.Sci.43. 642-645 (1993)

Miyoshi，I.，Yamashita，T.，Aoyama，S.，Sasaki，N.，Maki，K.，Kasai，N：“环境因素对激素诱导超卵化后获得的小鼠胚胎产量的影响”Lab.Anim。

Miyosi, I., Yamasita, Y., Aoyama, S., Sasaki, N., Maki, K., and Kasai, N.: "Effect of environmental factors on the yield of mouse embryos obtained after hormone-induced superovulation." Lab.Anim.Sci.43. 642-645 (1993)

Miyosi, I.、Yamasita, Y.、Aoyama, S.、Sasaki, N.、Maki, K. 和 Kasai, N.：“环境因素对激素诱导超排卵后获得的小鼠胚胎产量的影响。”