The establishment of the standard method for rat embryo-cryopreservation method and for the network.

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Vitrification method for rat embryo cryopresearvation has not been established yet. In addition, it is not clear which facilitles are cryopresearving the rat embryos. The purpose of this research is as follows ;1. The establishment of the standard method for rat embryo-cryopresear vation.2. The development of the vitrification method for rat embryos.3. The establishment of the effective method for obtaining rat embryos.4. The establishment of the database and the network system for rat embryo-cryopresearvation in Japan.The best method for rat embryo-cryopresearvation is the method that Mizuno et al. developed. They reported and we also think that the 2 cell embryos are the best stage for cryopresearvation.Hirabayashi et al. and Kasai et al. improved the vitrification methods previously reported. But the methods have not been completed.Ishikawa et al. and Yamazaki et al. improved the methods for obtaining rat embryo-cryopresearvation by the induced ovulation with PMSG and hCG and these methods are useful for practice.The database and the network for rat embryo-cryopresearvation have not been established yet in this study. We will continue to do these purpose.