权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The analyses of role of copper on the diabetes in the animal model with diabetes and copper metabolism deficiency

糖尿病铜代谢缺乏动物模型中铜对糖尿病的作用分析

基本信息

批准号：
18500324
负责人：
KASAI Noriyuki
金额：
$ 2.57万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Young LEA. LEC-Atp7b^<hts> congenic rats exhibit impaired PPI because of their lower brain copper contentsLong-Evans Cinnamon (LEC) rat is an animal model of human Wilson's disease and exhibit excessive accumulation of copper in liver. The LEC rat has a defect in Atp7b, which encodes a copper-transporting P-type ATPase. The LEA. LEC-Atp7b^<hts> congenic rat is Long-Evans Agouti (LEA) rat except for the defect in Atp7b from the LEC rat. LEA. LEC-Atp7b^<hts> rats from 4 to 8 weeks of age are very active therefore it is not easy to change cages, while LEA rats are not as active as LEA. LEC-Atp7b^<hts> rats.In this study, to characterize the behavioral phenotype in young LEA. LEC-Atp7b^<hts> rats, home cage activity test, open field test and prepulse inhibition (PPI) testwere carried out. And we measured brain copper contents. From 6 to 8 weeks old male LEA. LEC-Atp7b^<hts> rats were used for these experiments compared with LEA rats at the same age. Furthermore the LEA rats administrated D-penicillamine were used for PPI test and measurement of brain copper contents.As the results of these experiments LEA. LEC-Atp7b^<hts> rats exhibited significantly lower PPI than LEA rats and no difference in home cage activity test and open field test. And the brain copper content in LEA. LEC-Atp7b^<hts> rats was significantly lower than LEA rats. LEA rats administrated PA showed lower PPI and lower brain copper content as well as LEA. LEC-Atp7b^<hts> rats. These results indicate that the copper plays some roles in the brain and causes impaired PPI and the behavior in LEA. LEC-Atp7b^<hts> rats.

年轻的莱亚。LEC-Atp 7 b-1<hts>同源大鼠由于其较低的脑铜含量而表现出受损的PPI。Long-Evans Cinnamon（LEC）大鼠是人类Wilson病的动物模型，并且表现出铜在肝脏中的过度积累。LEC大鼠在Atp 7 b中存在缺陷，Atp 7 b编码铜转运P型ATP酶。莱亚。LEC-Atp 7 b-<hts>同源大鼠是Long-Evans Agglutinus（莱亚）大鼠，除了来自LEC大鼠的Atp 7 b缺陷之外。莱亚。4 <hts>- 8周龄的LEC-Atp 7 b ^大鼠非常活跃，因此不容易更换笼子，而莱亚大鼠不如莱亚活跃。在<hts>这项研究中，为了表征年轻莱亚的行为表型。对LEC-Atp 7 b ^<hts>大鼠进行笼内活动实验、旷场实验和前脉冲抑制实验。我们测量了脑铜含量。6 - 8周龄雄性莱亚。LEC-Atp 7 b ^<hts>大鼠用于这些实验，并与相同年龄的莱亚大鼠进行比较。此外，对给予D-青霉胺的莱亚大鼠进行PPI试验和脑铜含量测定。在<hts>笼内活动试验和旷场试验中，LEC-Atp 7 b ^大鼠的PPI显著低于莱亚大鼠，但无差异。莱亚脑铜含量。LEC-Atp 7 b ^<hts>大鼠明显低于莱亚大鼠。莱亚大鼠给予PA后，PPI和脑铜含量均降低，莱亚也降低。LEC-Atp 7 b ^<hts>大鼠。这些结果表明，铜在脑中发挥一定作用，并导致PPI受损和莱亚的行为。LEC-Atp 7 b ^<hts>大鼠。