A Research for cryopreservation and transportation of embryos of small lsboratory animals.

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We attempted to develop two embryo transport systems using nonfreezing techniques and using a combination of vitrification techniques and a non-liquid type liquid nitrogen container. For nonfreezing technique, mouse morulae were kept in 0.5M Sucrose-PB1 solution in a thermos bottle with ice-water, followed by packaging with polystyrene foam. For vitrification technique, mouse morulae were kept in 40% ethylene glycol, 30% Ficoll and 0.5M sucrose in a 0.25ml plastic straw, followd by immediate immersing in liquid nitrogen and packaging in a non-liquid type ultralow temperature container with a special shipping enclosure. The packages were transported from Kochi to Sapporo, about 1300km by a forwarding agent. All packages arrived at the destination in 48 hours. After shipping, the survival of embryos was 46.8% for nonfreezing technique and 88.0% for vitrification techniques when evaluated by development to blastocysts in vitro. A final birthrate was 14.1% and 45.8% for nonfreezing techniques and vitrification techniques, respectively. Both systems are cheap, convenient and practical for embryo transports, and can be selected by the situation of sending and receiving facilities.We also attempted to improved the preservation technique by vitrification for mouse embryos and rat embryos. These improved preservation techniques are very simple and much helpful for prevailling embryo preservation and transport system.We also showed embryo transfer technique can clear up of infections organisms from conventional animals, indicating that we may disregard the difference of microbiogical level between the donor and recipient laboratories owing to the intrinsic aseptic condition of treated embryos.