Production and Charactrerization of New Conjenic strain of LEC rats which is a Model for Spontaneous Hepatits and Hepatoma

自发性肝炎和肝癌模型LEC大鼠新Conjenic品系的产生和表征

• 批准号: 07558237
• 负责人: KASAI Noriyuki
• 金额: $ 0.7万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558237/
• 关键词: LEC rats; Wilson disease; P-type ATPase; hepatitis(hts)gene; LEA rats; backcross; jaundice; LEA・LEC-hts conjenic rats; 自然発症肝炎肝癌ラット; ウイルソン病; セルロプラスミン; 肝炎遺伝子; コンジェニックラット

LEC rats, a model of human Wilson disease, have been revealed that not only their clinical aspects but also gene level were idenical with human Wilson disease, which gene was encoded with P-type ATPase. On the way of establishing the LEC rat, control strain, of which genetic trait is identical with the LEC rat except for the locus of hepatitis gene, hts, was not maintained. Therefore, we tried to establish conjenic strain from LEA rats by backcross of hts gene of LEC rats. For three and half years, eight times of backcross (N8) were performed and established the conjenic strain named LEA・LEC-hts. All of female rats, N8F1, with homogeneous hts genes died with jaundice at the age of 19 to 20 weeks whereas half of male rats did at the age of 20 to 21 weeks. Theoretically, the rate of replacement of background gene is 99.2% on the cromosome without hts gene by eght times of backcross, 52.2% at 10cM distant from the hts gene, and 6.8% at 1cM distant from the hts gene.As LEA rats of a background strain have been used most frequently as control of LEC rats, backround data are piled up much.Therefore, LEA・LEC-hts conjenic rats arevery useful for reseach of Wilson disease or hepatits and hepatoma of LEC rats.

LEC大鼠作为人Wilson病的模型，不仅在临床表现上，而且在基因水平上都与人Wilson病一致，该基因编码p型atp酶。在建立LEC大鼠的过程中，没有维持除肝炎基因位点hts外与LEC大鼠遗传性状相同的对照菌株。因此，我们试图通过LEC大鼠的hts基因回交，建立LEA大鼠的遗传菌株。在3年半的时间里，进行了8次回交（N8），建立了LEA·LEC-hts传染菌株。所有具有同种基因的雌性大鼠N8F1在19至20周龄死于黄疸，而雄性大鼠有一半在20至21周龄死于黄疸。理论上，无hts基因的染色体经过8次回交，背景基因的置换率为99.2%，距离hts基因10cM处置换率为52.2%，距离hts基因1cM处置换率为6.8%。由于一种背景品系的LEA大鼠作为LEC大鼠的对照使用最为频繁，因此背景数据堆积较多。因此，LEA·LEC-hts遗传大鼠对肝豆状核变性或LEC大鼠的肝脏和肝癌的研究非常有用。

项目成果

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Inanami O.：“通过服用路易波士茶（Aspalathus Linearis）来抑制大鼠大脑中与年龄相关的脂质过氧化物的积累。”

Inanami O., Asanuma T., Inukai N,Jin S., Shimokawa S., Kasai N., Nakano M., Sato F., and Kuwabawa M.: "The suppression of agerelated accumulation of lipid peroxides in rat brain by administration of Rooibos tea(Aspalathus linearis)." NeuroSci.Letters. 196

Inanami O.、Asanuma T.、Inukai N、Jin S.、Shimokawa S.、Kasai N.、Nakano M.、Sato F. 和 Kuwabawa M.：“通过给药抑制大鼠脑中与年龄相关的脂质过氧化物的积累

Nakayama K.et al: "Visualization of yellowish-orange Luminescence from caprous metallothioneins in liver of Long-Evans Cinnamon rats" Biochem.Biophys.Acta. 1289. 150-158 (1996)

Nakayama K.等人：“长埃文斯肉桂大鼠肝脏中羊皮金属硫蛋白黄橙色发光的可视化”Biochem.Biophys.Acta。

Ariga.T.etal: "Accumulation of isoglobosidc and ganglio-N-tetraosyl ceraptide having blood group B deter minant in hepatomas of fcmale LECrats" Biochem.Biophys.Acta. 1254. 257-266 (1995)

Ariga.T.etal：“在雄性 LECrat 的肝癌中具有 B 型血决定因素的异球苷和神经节-N-四糖基塞肽的积累”Biochem.Biophys.Acta。