Production and Charactrerization of New Conjenic strain of LEC rats which is a Model for Spontaneous Hepatits and Hepatoma

LEC大鼠，人类威尔逊病的一种模型，已经被揭示出来，不仅是其临床方面，而且基因水平与人类威尔逊病相同，基因被编码为P型ATP酶。在建立LEC大鼠、控制链的方法中，遗传特征的方法是确定的，除了LEC大鼠在肝炎基因的位置， hts，但没有维护。因此，我们试图从LEA老鼠身上建立一条可靠的脊椎神经，由hts的LEC老鼠的基因组成。在三个半年的时间里，八次背交叉（N8）已经被证明和建立了命名为LEA-LEC-hts的conjenic strain。所有的女老鼠，N8 F1，和同源的hts的基因在19至20周的时候死于Jaundice，一半的女老鼠在20至21周的时候死于20至21周的时候。理论上，背景基因的替换率在99.2%的情况下没有hts基因的背交叉时间，52.2%从hts基因的10 cm距离，6.8%从hts基因的1cM距离。作为一只LEA老鼠的背景紧张症经常被用作控制LEC老鼠，背景数据被编译得太多。因此，LEA-LEC-hts conjenic rats被用来研究威尔逊疾病或肝炎和LEC rats的Hepatoma。

LEC rats, a model of human Wilson disease, have been revealed that not only their clinical aspects but also gene level were idenical with human Wilson disease, which gene was encoded with P-type ATPase. On the way of establishing the LEC rat, control strain, of which genetic trait is identical with the LEC rat except for the locus of hepatitis gene, hts, was not maintained. Therefore, we tried to establish conjenic strain from LEA rats by backcross of hts gene of LEC rats. For three and half years, eight times of backcross (N8) were performed and established the conjenic strain named LEA・LEC-hts. All of female rats, N8F1, with homogeneous hts genes died with jaundice at the age of 19 to 20 weeks whereas half of male rats did at the age of 20 to 21 weeks. Theoretically, the rate of replacement of background gene is 99.2% on the cromosome without hts gene by eght times of backcross, 52.2% at 10cM distant from the hts gene, and 6.8% at 1cM distant from the hts gene.As LEA rats of a background strain have been used most frequently as control of LEC rats, backround data are piled up much.Therefore, LEA・LEC-hts conjenic rats arevery useful for reseach of Wilson disease or hepatits and hepatoma of LEC rats.