Regulation of Gene Expression

点击翻译按钮获取中文摘要

Following results were obtained :1. We isolated several homologues of Vp-1 or Abi 3 genes which are essential for ABA-mediated signal transduction in the regulation of higher plant morphogenesis.2. By using gene targeting, we investigated function of several genes, including mel-18 which is a negative regulatory transcription factor and controls the expression of homeobox genes. Mel-18 knock-outs showed posterior transformation of skeletal bones, suggesting their equivalent functions to those of Drosophila.3. By NMR,molecular interactions between IgG and protein-A,-G,and -L,were analyzed. It was revealed that 1) the IgG-binding domains of protein-A and -G have quite different three-dimensional structures, but bind to sites on the Fc that overlap extensively, 2) protein G employs two quite different regions of its surface to bind to the Fab and the Fc regions, and 3) the fold of the IgG-binding domain of protein L is similar to that of protein G,but they bind to a different domain in the Fab.4. By indirect immunofluorescence microscopy, we analyzed location and morphological change of chromatin region and microtuble especially during mitosis in the fission yeast S.pombe cells.5. For development of high sensitive biosensor, some devices with the chamber packing beads immobilized with microorganisms were fabricated by micromachine and CCD camera system. The system gave current response proportional to glucose concentration. Distribution of the luminescendce gave theoretical decrease as a function of low-rate and activity of immobilized enzyme. The system has ability to be highly sensitive due to chemical amplification by the enzyme induced by analyte.6. We discussed future collaborations on France-Japan Immunology, particularly on T-B collaboration, regulation lymphocyte development, and immune diseases. Collaboration includes exchange of information, materials, and young scientists.