Molecular analysis of T cell antigen receptors and functional molecules.

利用BW5147和C57BL/6 KLH-Ts融合制备的可诱导的KLH特异性抗Id -Ts杂交瘤，分析了抗独特型受体（Id）对抑制性T细胞（Ts）的免疫作用及其分子遗传学特性。以下是观察结果和结论。抗- id - t受体是外部抗原的调节内部图像（立体图像），主要由抑制家族看到。通过以下四项实验获得了内部图像由Ts受体V区组成的证据：a) Anti-Id Ts杂交瘤在细胞表面表达外部抗原表位（klh样决定因子）的立体拷贝。b)特定的抗KLH抗体与立体拷贝发生反应，并具有抑制KLH特异性和H- <2^b>限制性反应的活性。c)嵌合体条件下，Ts上KLH样决定因子的表型表达发生改变。d)大鼠抗id - Ts杂交瘤抗体与KLH和KLH特异性和介导的H- <2^b>限制性抑制活性发生反应。利用x射线照射骨髓嵌合体解决了I-J悖论。在Ts.4上发现I-J行列式是独特型结构。FPLC系统分析了来自抗id Ts杂交瘤的抑制因子，发现其为30 KD分子，pI为6.1 ~ 6.2.5。我们还成功克隆和测序了在Ts杂交瘤中具有活性的Ts α链基因（cDNA）。然而，Ts受体分子似乎与Th和Tc上的受体分子具有不同的构型，因为Ts杂交瘤中的Ts β链基因都被删除了。提出一个未定义的第四链与Ts α链存在关联。

We attempted to analyse immunological roles of anti-idiotypic (Id) receptor on suppressor T cells (Ts) and their molecular genetic properties by using inducible KLH specific anti-Id Ts hybridoma make by fusion of BW5147 and C57BL/6 KLH-Ts. The following observations and conclusions were oftained.1. Anti-Id Ts receptor works as an regulatory internal image (stereocopy) of the external antigen predominantly seen by suppressor families.2. The evidence that the internal image is composed of the Ts receptor V region is obtained by the following four experiments:a) Anti-Id Ts hybridoma expresses the stereocopy of the external antigenic epitope (KLH-like determinant) on the cell surface.b) The particular anti-KLH antibody reacts with the stereocopy and also possesses the activity to suppress the responses in the KLH specific and H- <2^b> restricted manner.c) The phenotypic expression of the KLH-like determinant on Ts is changed under chimera conditions.d) The rat antibody against anti-Id Ts hybridoma reacts with KLH and KLH-specific and mediated H- <2^b> restricted suppressor activity.3. The paradox of I-J has been solved by using x-irradiation bone marrow chimera. The I-J determinant is found to be the idiotypic structure on Ts.4. The suppressor factor derived from anti-Id Ts hybridoma is analysed by FPLC system and found to be the 30 KD molecule with pI 6.1-6.2.5. We have also been successful in cloning and sequencing of Ts alpha chain gene (cDNA) which is found to be active in Ts hybridomas. However, Ts receptor molecules seem to have different configulation from those on Th and Tc, because Ts beta chain genes in Ts hybridomas are all deleted. An undefined forth chain is proposed to exist in association with Ts alpha chain.