Tertiary structure and molecular modeling of insect neuropeptides

昆虫神经肽的三级结构和分子模型

• 批准号: 05403031
• 负责人: SUZUKI Akinori
• 金额: $ 24.83万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05403031/
• 关键词: NMR; Tertiary structure; PTTH; Insulin; インスリン族ペプチド; ボンビキシン; 昆虫神経ペプチド; 前胸腺刺激ホルモン; 昆虫; ペプチド

1.The tertiary structure of bombyxin was determined by the NMR method.2.From the bioassay of chimeric molecules of bombyxin and insulin, the central part of B-chain, TyrB6-LeuB18, is found to be very important for bombyxin activity.3.Solution structure determination of these chimeric molecules revealed that their B-chain central parts took similar main-chain conformation, but formed dissimilar patches on their molecular surface. Therefore, the surface patch formed by the central part of the bombyxin B-chain is of critical importance for recognition of bombyxin receptor.4.NMR data of PTTH revealed that the central part of PTTH has rigid structure and is rich in beta-sheet.5.Molecular modeling of PTTH by computer suggested that PTTH has two b-sheets and is belong to the cystine knot superfamily.

1.用核磁共振法测定了蚕蛹素的三级结构。通过对蚕蚕素与胰岛素嵌合分子的生物测定，发现b链的中心部分TyrB6-LeuB18对蚕蚕素的活性起重要作用。对这些嵌合分子的溶液结构测定表明，它们的b链中心部分具有相似的主链构象，但在分子表面形成不同的斑块。因此，蚕蛹素b链中心部分形成的表面斑块对蚕蛹素受体的识别至关重要。PTTH的核磁共振数据显示，PTTH的中心部分具有刚性结构，富含β -薄片。PTTH的计算机分子模拟表明，PTTH有两个b-片，属于胱氨酸结超家族。

项目成果

Nagata: "Insect prothoracocotropic hormone : a new menber of the vertebrate growth factor superfamily." Peptide Chemistry 1994. 85-88 (1995)

Nagata：“昆虫促促胸腺激素：脊椎动物生长因子超家族的新成员。”

T.Noguchi: "Three-dimensional structure and receptor-recognition surface of Bombyxin-II,an insulin-related peptide of the silkmoth Bombyx mori." FEBS Letter. 376. 251-256 (1995)

T.Noguchi：“Bombyxin-II 的三维结构和受体识别表面，Bombyxin-II 是蚕蛾 Bombyx mori 的胰岛素相关肽。”

J.Ishibashi: "Determination of disulfide bond location of Bombyx prothoracicotropic hormone,a homodimeric peptide." Peptide Chemistry 1992. 568-571 (1993)

J.Ishibashi：“测定家蚕促胸腺激素（一种同二聚肽）的二硫键位置。”

J.Ishibashi: "Assignment of disulfide bond location in prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, a heterodimeric peptide." Biochemistry. 33. 5912-5919 (1994)

J.Ishibashi：“家蚕促促胸腺激素 (PTTH) 中二硫键位置的分配，Bombyx mori，一种异二聚肽。”

K.Nagata: "Three-dimensional structure of bombyxin-II,an insulin-like peptide of the silkmoth Bombyx mori. Structural comparison with insulin and relaxin." J.Mol.Biol.253. 749-758 (1995)

K.Nagata：“蚕素-II 的三维结构，这是一种来自家蚕的胰岛素样肽。与胰岛素和松弛素的结构比较。”