Tertiary structure and molecular modeling of insect neuropeptides

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1.The tertiary structure of bombyxin was determined by the NMR method.2.From the bioassay of chimeric molecules of bombyxin and insulin, the central part of B-chain, TyrB6-LeuB18, is found to be very important for bombyxin activity.3.Solution structure determination of these chimeric molecules revealed that their B-chain central parts took similar main-chain conformation, but formed dissimilar patches on their molecular surface. Therefore, the surface patch formed by the central part of the bombyxin B-chain is of critical importance for recognition of bombyxin receptor.4.NMR data of PTTH revealed that the central part of PTTH has rigid structure and is rich in beta-sheet.5.Molecular modeling of PTTH by computer suggested that PTTH has two b-sheets and is belong to the cystine knot superfamily.