癌組織核DNA量測定による抗癌剤効果判定の試み

尝试通过测量癌组织中核DNA的量来确定抗癌药物的有效性

• 批准号: 05770945
• 负责人: 忠岡 信彦
• 金额: $ 0.45万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05770945/
• 关键词: DNA ploidy; 化学療法; 胃癌; 化学療法効果判定

手術を前提とした消化器癌(Borrmann 4型進行胃癌)患者20名を対象とした。UFT・CDDP療法に準じて手術6週間前より抗癌剤投与を開始した。(UFT400mg/m^2×42,CDDP80mg/m^2×2)。抗癌剤投与開始前、投与中(内視鏡下)、投与後(手術標本)に検体組織を採取した。これよりFlow cytometryを用いて細胞核DNA量を測定した。さらに組織内5-FU濃度、TS阻害を測定した。HE染色標本を作製し、病理組織学的効果判定を実施した。核DNA量は全例で測定が可能であり、1症例あたり3〜4回、計72検体の測定を実施した。組織内5-FU濃度、TS阻害率を測定ではそれぞれ11症例が評価可能であった。得られたhistogramよりDNApattern,cell cycleの変化をみ、組織内抗癌剤濃度、組織学的効果、臨床効果との関連について検討し、以下の結果を得た。1.化学療法後のDNA ploidy pattern変化はdiploid例で%S期細胞比の増大、aneuploid例でaneuploidpeakの平坦化としてとらえられ、化学療法効果が大きいほどその変化も大きかった。2.DNA pattern変化と組織学的効果とは相関が認められ臨床効果を反映した。3.組織5-FU濃度、TS量、TS阻害率と組織学的効果に関連は認められなかった。4.抗腫瘍効果指標としてのDNA ploidy pattern分析の有用性が示唆された。上記内容の一部を第52回日本癌学会総会(演題番号2367)で発表した。また論文として癌と化学療法誌に投稿中である。

The prerequisite for surgery was 20 patients with digestive system cancer (Borrmann type 4 gastric cancer). UFT・CDDP therapy was accurate and anti-cancer medication was started 6 weeks before the surgery. (UFT400mg/m^2×42, CDDP80mg/m^2×2). Body tissues are collected before, during, and after the administration of anticancer drugs (endoscopy) (surgical specimens).これよりFlow cytometry is used to measure the amount of nuclear DNA in いて. It is used to measure the concentration of 5-FU in tissues and the inhibition of TS. HE staining specimens were prepared and the results of pathological histology were determined. It is possible to measure the amount of nuclear DNA in all cases, 3 to 4 times per case, and 72 cases in total. The concentration of 5-FU in the tissue and the inhibition rate of TS were measured and evaluated in 11 cases. The following results were obtained based on the DNA pattern, cell cycle changes, anti-cancer agent concentration in the tissue, histological effects, and clinical effects. 1.DNA ploidy after chemotherapy Pattern change means that the cell ratio in S phase is increased, aneuploid example means that the cell ratio in S phase is increased, aneuploid example means that the cell ratio is flattened, and the effect of chemotherapy is large. 2. The histological effect of DNA pattern modification is related to the clinical effect and reflects the clinical effect. 3. Tissue 5-FU concentration, TS amount, TS inhibition rate are closely related to the histological effect. 4. The usefulness of DNA ploidy pattern analysis as an indicator of anti-tumor effect is demonstrated. The above content is part of the 52nd Japanese Cancer Society Meeting (title number 2367). The paper "Cancer and Chemotherapy Chronicles" is currently being submitted.