Geographic Pattern of the Microsatellite DNA Polymorphism in two species of Sorex shrew in Hokkaido

北海道两种鼩鼱微卫星DNA多态性的地理格局

• 批准号: 13839002
• 负责人: OHDACHI Satoshi
• 金额: $ 1.86万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13839002/
• 关键词: Shrew; Microsatellite DNA; Cytochrome b; Genetic structure; Northeastern Asia; 個体群; 北海道

The biogeographic history of six soricine shrew species inhabiting the Japan archipelago and neighboring regions has been studied based on intra- and interspecific phylogeny of nucleotide sequences of mitochondrial cytochrome b gene.To infer biogeographic histories of S. caecutiens and S. unguiculatus in more detail, phylogenetic relationships between species or populations were investigated by restriction fragment length polymorphism (RFLP) for the nuclear ribosomal RNA genes (rDNA). Genetic population structures were examined using microsatellite DNA markers.From the rDNA-RFLP analysis, it was suggested in S. caecutiens that Hokkaido population is genetically distant from Eurasian ones and Sakhalin population originated from hybridization between the continent and Hokkaido populations.High genetic variations in microsatellite loci were observed among Hokkaido mainland populations of both species. The allelic richness and heterozygosity were proportional to the island size and positiv … More e correlation between genetic and geographic distances was detected in S. unguiculatus, while S. caecutiens showed no positive correlation between genetic and geographic distances among local populations in Hokkaido mainland. This implies that the final population expansion in Hokkaido occurred in S. unguiculatus earlier than in S. caecutiens.Combining the information from rDNA-RFLP, microsatellite DNA and mtDNA cyt-b, biogeographic histories of S. unguiculatus and S. caecutiens in Hokkaido were inferred as follows: Sorex caecutiens, which invaded Hokkaido before the last glacial age, was restricted to the forest refuge in southern Hokkaido because of its sylvicolous nature, at the maximum stage of last glacial age, where grassland vegetation prevailed in Hokkaido. However, it expanded its range with recovery of forest vegetation all over Hokkaido in post glacial age. S. unguiculatus invaded Hokkaido later than S. caecutiens, probably at the maximum stage of last gracial age, and soon came distributed throughout Hokkaido inhabiting not only the southern forest refuge but also grasslands prevaily under the cold climate. Less

基于线粒体细胞色素b基因的种内和种间系统发育关系，研究了生活在日本群岛及其邻近地区的6种山梨鼠的生物地理史。为了更详细地推测其生物地理史，利用核糖体RNA基因(RDNA)的限制性片段长度多态性(RFLP)研究了物种或种群之间的系统发育关系。利用微卫星DNA分子标记分析了北海道种群和库页岛种群的遗传结构。rDNA-RFLP分析表明，北海道种群与欧亚种群遗传距离较远，库页岛种群起源于大陆种群与北海道种群的杂交。两个物种的北海道大陆种群在微卫星座位上均存在较高的遗传变异。等位基因丰富度和杂合度与岛屿大小和正…成正比长爪沙鼠的遗传距离与地理距离之间的相关性较强，而北海道本土种群间的遗传距离与地理距离之间无正相关关系。结合rDNA-RFLP、微卫星DNA和线粒体DNA Cyt-b的信息，推测北海道长爪沙鼠和长尾沙鼠的生物地理史如下：北海道最后一次冰期前入侵北海道的长爪沙鼠Sorex caecutiens仅限于北海道南部的森林保护区，因为其高山性质，在末次冰期最盛期，北海道草原植被占优势。然而，随着后冰川时代北海道各地森林植被的恢复，它的范围扩大了。长爪沙鼠入侵北海道较晚，可能在末次优势期的盛期，很快就分布在北海道各地，不仅栖息在南部森林保护区，而且在寒冷的气候下普遍分布在草原上。较少

项目成果

Nesterenko, V., S.D.Ohdachi: "Postnatal growth and development in Sorex unguiculatus"Mammal Study. 26. 145-148 (2001)

Nesterenko, V., S.D.Ohdachi：“Sorex unguiculatus 的产后生长和发育”哺乳动物研究。

Naitoh Y, Y. Ishibashi, S. Abe, S. D. Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

Naitoh Y、Y. Ishibashi、S. Abe、S. D. Ohdachi：“Sorex unguiculatus 和 S. caecutiens 两种鼩鼱多态性微卫星 DNA 标记的分离和表征”分子生态学笔记。

Naitoh, Y, Y.Ishibashi, S.Abe, S.D.Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

Naitoh, Y, Y.Ishibashi, S.Abe, S.D.Ohdachi：“两个鼩鼱物种（Sorex unguiculatus 和 S. caecutiens）多态性微卫星 DNA 标记的分离和表征”分子生态学笔记。

Ohdachi, S.D., M.A.Iwasa, V.A.Nesterenko, H.Abe, R.Masuda, W.Haberl: "Molecular Phylogenetics of Crocidura shrews (Insectivora) in East and Central Asia"Journal of Mammalogy. (印刷中).

Ohdachi, S.D.、M.A.Iwasa、V.A.Nesterenko、H.Abe、R.Masuda、W.Haberl：“东亚和中亚食虫目鼩鼱的分子系统发育”《哺乳动物学杂志》（正在出版）。

Han S. -H., M. A. Iwasa, S. D. Ohdachi, H. -S. Oh, H. Suzuki, K. Tsuchiya, H. Abe: "Molecular phylogeny of Crocidura shrews in northeastern Asia: a special reference to specimens on Cheju Island, South Korea"Acta Theriologica. 47. 369-379 (2002)

Han S. -H.，M. A. Iwasa，S. D. Ohdachi，H. -S。