Geographic Pattern of the Microsatellite DNA Polymorphism in two species of Sorex shrew in Hokkaido

北海道两种鼩鼱微卫星DNA多态性的地理格局

• 批准号: 13839002
• 负责人: OHDACHI Satoshi
• 金额: $ 1.86万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13839002/
• 关键词: Shrew; Microsatellite DNA; Cytochrome b; Genetic structure; Northeastern Asia; 個体群; 北海道

The biogeographic history of six soricine shrew species inhabiting the Japan archipelago and neighboring regions has been studied based on intra- and interspecific phylogeny of nucleotide sequences of mitochondrial cytochrome b gene.To infer biogeographic histories of S. caecutiens and S. unguiculatus in more detail, phylogenetic relationships between species or populations were investigated by restriction fragment length polymorphism (RFLP) for the nuclear ribosomal RNA genes (rDNA). Genetic population structures were examined using microsatellite DNA markers.From the rDNA-RFLP analysis, it was suggested in S. caecutiens that Hokkaido population is genetically distant from Eurasian ones and Sakhalin population originated from hybridization between the continent and Hokkaido populations.High genetic variations in microsatellite loci were observed among Hokkaido mainland populations of both species. The allelic richness and heterozygosity were proportional to the island size and positiv … More e correlation between genetic and geographic distances was detected in S. unguiculatus, while S. caecutiens showed no positive correlation between genetic and geographic distances among local populations in Hokkaido mainland. This implies that the final population expansion in Hokkaido occurred in S. unguiculatus earlier than in S. caecutiens.Combining the information from rDNA-RFLP, microsatellite DNA and mtDNA cyt-b, biogeographic histories of S. unguiculatus and S. caecutiens in Hokkaido were inferred as follows: Sorex caecutiens, which invaded Hokkaido before the last glacial age, was restricted to the forest refuge in southern Hokkaido because of its sylvicolous nature, at the maximum stage of last glacial age, where grassland vegetation prevailed in Hokkaido. However, it expanded its range with recovery of forest vegetation all over Hokkaido in post glacial age. S. unguiculatus invaded Hokkaido later than S. caecutiens, probably at the maximum stage of last gracial age, and soon came distributed throughout Hokkaido inhabiting not only the southern forest refuge but also grasslands prevaily under the cold climate. Less

已经研究了居住在日本群岛和邻近地区的六种s虫的生物地理历史，该物种是基于线粒体细胞色素b gene的核肽序列的内部和特定的系统发育研究的研究核核糖体RNA基因（RDNA）的多态性（RFLP）。使用微卫星DNA标记对遗传种群结构进行了检查。从rDNA-RFLP分析中，在S. caecutiens中提出，北海道人口与欧亚大陆的人群在遗传上相距较远，而萨卡林人口则源自大陆和北海道种群之间的杂交。等位基因的丰富度和杂合性与岛屿的大小成正比……在非构菌链球菌中检测到了遗传和地理距离之间的更多相关性，而苏氏链球菌在北海道大陆的局部人群之间没有遗传和地理之间的正相关。这意味着，北海道的最终人口扩大发生在S. unguiculatus中，而不是在S. caecutiens.com.com.com.bandna-rflp，微卫星DNA和mtDNA Cyt-b，S。nogiculatus和S. caecutiens的生物地理学历史，北海岸的S. caikaido selly aundere the socts caicuti：冰川时代由于其造成的性质，在最后冰川时代的最大阶段，在北海道南部的森林避难所仅限于森林避难所，那里的草原植被在北海道盛行。但是，它随着冰川后时代的整个北海道的恢复而扩大了其范围。 S. unguiculatus比Caecutiens晚了北海道，可能处于上一个粮食时代的最大阶段，很快就在整个北海道分发，不仅居住在南部森林避难所，而且在寒冷的气候下占据了草地。较少的

项目成果

Nesterenko, V., S.D.Ohdachi: "Postnatal growth and development in Sorex unguiculatus"Mammal Study. 26. 145-148 (2001)

Nesterenko, V., S.D.Ohdachi：“Sorex unguiculatus 的产后生长和发育”哺乳动物研究。

Naitoh Y, Y. Ishibashi, S. Abe, S. D. Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

Naitoh Y、Y. Ishibashi、S. Abe、S. D. Ohdachi：“Sorex unguiculatus 和 S. caecutiens 两种鼩鼱多态性微卫星 DNA 标记的分离和表征”分子生态学笔记。

Ohdachi, S.D., M.A.Iwasa, V.A.Nesterenko, H.Abe, R.Masuda, W.Haberl: "Molecular Phylogenetics of Crocidura shrews (Insectivora) in East and Central Asia"Journal of Mammalogy. (印刷中).

Ohdachi, S.D.、M.A.Iwasa、V.A.Nesterenko、H.Abe、R.Masuda、W.Haberl：“东亚和中亚食虫目鼩鼱的分子系统发育”《哺乳动物学杂志》（正在出版）。

Naitoh, Y, Y.Ishibashi, S.Abe, S.D.Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

Naitoh, Y, Y.Ishibashi, S.Abe, S.D.Ohdachi：“两个鼩鼱物种（Sorex unguiculatus 和 S. caecutiens）多态性微卫星 DNA 标记的分离和表征”分子生态学笔记。

Han S. -H., M. A. Iwasa, S. D. Ohdachi, H. -S. Oh, H. Suzuki, K. Tsuchiya, H. Abe: "Molecular phylogeny of Crocidura shrews in northeastern Asia: a special reference to specimens on Cheju Island, South Korea"Acta Theriologica. 47. 369-379 (2002)

Han S. -H.，M. A. Iwasa，S. D. Ohdachi，H. -S。