Comperative study in the viability of the liver graft harvested from cadaver and living donor, evaluated by assessment of tissue oxygenation in sinusoid and oxidation of hepatocyte

通过评估肝窦组织氧合和肝细胞氧化来评估从尸体和活体供体中获取的肝移植物的活力的比较研究

• 批准号: 06044127
• 负责人: YAMAOKA Yoshio
• 金额: $ 15.1万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044127/
• 关键词: Liver TRASPLANTATION; REGECTION; Near infra-red spectroscopy; micro circulation; hepaticsinusoid; oxygen satulation; primary-nonfunction; 近赤外光分析; 生体部分肝移植; 脳死肝移植

As hemoglobin can be used as an optical parameter of microcirculation, oxygen saturation and content of hemoglobin of red cells in the liver sinusoids can be evaluated with tissue near-infrared spectroscopy. Using this novel method, we have studied relationship between the circulation status and post-transplantation pathophisiology. Grafts that presented aspiration cytology scores of 2 or more at 1 week after operation (groupI, n=14) were associated with a higher heterogeneity than grafts with no or very mild intragraft immune activation (group II,n=33). Group I was also accompanied by higher postoperative peak GOT level and higher donor age. Heterogenous reperfusion, higher peak GOT level and higher donor age were also associated with clinical rejection at 1week, but not with later-onset rejection.

由于血红蛋白可以作为微循环的光学参数，因此可以用组织近红外光谱分析来评估肝窦内红细胞的血氧饱和度和血红蛋白含量。利用这一新方法，我们研究了血液循环状态与移植后病理的关系。术后1周抽吸细胞学评分大于或等于2的移植物(n=14)与移植物内无或非常轻微免疫激活的移植物(n=33)相比具有更高的异质性。I组术后GOT峰值较高，供者年龄较高。异种再灌注、高谷草转氨酶水平和供者年龄与术后1周的临床排斥反应有关，但与较晚发生的排斥反应无关。

项目成果

N.Yanabu: "Evaluation of circulation status tissue near-infrared spectroscopy in living related liver transplantation across ABO blood groups." Transprantation Proceedings. 27(1). 1160-1163 (1995)

N.Yanabu：“跨 ABO 血型的活体相关肝移植中循环状态组织近红外光谱的评估。”

K.Tanaka: "Living related liver transplantation in children." The American Journal of Surgery. 168. 41-48 (1994)

K.Tanaka：“儿童活体相关肝移植。”

T.Kiuchi: "Implications of host pancreatic hormones in the restart of grafted liver." Hepatology. 21 (6). 1561-1567 (1995)

T.Kiuchi：“宿主胰腺激素对移植肝脏重启的影响。”