Perioperative management in clinical liver traus plantation

临床肝外伤种植术的围手术期处理

• 批准号: 04044098
• 负责人: YAMAOKA Yoshio
• 金额: $ 15.36万
• 依托单位: Kyoto University Faculty of Medicine, The Secoud Department of Surgery
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04044098/
• 关键词: Liver Transplantation; Rejection; Lymphocyte MTT assay; Arterial Ketone Body Ratio (AKBR); 血中ケトン体比; リンパ球MTT活性; 病理学的検討

Perioperative management in clinical liver transplantationHistological changes comparing with energy metabolic changes in the rejection state after liver transplantation have not ever studied. In this plan, we have tried the MTT assay of lymphocyte comparing with the changes in arterial ketone body ratio (AKBR) which reflects the energy state of the liver mitochondria after liver transplantation in Hannover Medical Center. The activity of MTT decreased not only in the rejection state but also in the infectious state after liver transplantation. Therefore, MTT assay can not be good indicator of the rejection. However, in the patient with critically decreased in AKBR the MTT activity also decreased.As the collaboration study we have been evaluating measuring the saturation ratio of hemoglobin through the liver graft just after reperfusion using the MCPD method.

临床肝移植围手术期处理肝移植术后排斥反应状态下肝组织学变化与能量代谢变化的对比研究尚未见报道。本研究拟在德国汉诺威医学中心进行肝移植术后淋巴细胞MTT法检测，并与反映肝线粒体能量状态的动脉血酮体比值（AKBR）的变化进行比较。肝移植术后，MTT活性不仅在排斥状态下下降，而且在感染状态下也下降。因此，MTT法不能作为排斥反应的良好指标。然而，在AKBR严重降低的患者中，MTT活性也降低。作为合作研究，我们使用MCPD方法评估了在再灌注后即刻通过肝移植物测量血红蛋白饱和率。

项目成果

N.Ozaki: "Postoperative recovery of mitochondrial function of human liver graft procured and preserved with University of Wisconsin(UW)solution" Transplant.Int.3. 128-132 (1990)

N.Ozaki：“用威斯康星大学 (UW) 解决方案获得和保存的人肝移植物的线粒体功能术后恢复”Transplant.Int.3。

N.Ozaki: "Postoperative recovery of mitochondria function of human liver graft procured and preserved with University of Wisconsin (UW) Solution" Transplant.Int. 3. 128-132 (1990)

N.Ozaki：“使用威斯康星大学 (UW) 解决方案获得和保存的人肝移植物的线粒体功能术后恢复”Transplant.Int。

Y.Yamaoka: "Evaluation of the liver graft before procurement.Significance of arterial ketone body ratio in brain-dead patients" Transplant.Int.3. 78-81 (1990)

Y.Yamaoka：“移植肝移植前的评估。脑死亡患者动脉酮体比率的意义”Transplant.Int.3。

N.Ozaki: "Changes in energy substrates in relation to arterial ketone body ratio after human orthotopic liver transplantation" Surgery. 113. 403-409 (1993)

N.Ozaki：“人原位肝移植后能量底物与动脉酮体比率的变化”手术。

Y.Taki: "Significance of arterial ketone body ratio measurement in human liver transplantation" Transplantation. 49. 535-539 (1990)

Y.Taki：“动脉酮体比率测量在人体肝移植中的意义”移植。