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Eukaryotic Cell Cycle Control

真核细胞周期控制

基本信息

批准号：
06404020
负责人：
OKAYAMA Hiroto
金额：
$ 16.32万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06404020/
关键词：
cell cycle fission yeast ORC G1 phase S phase cdc2 cyclin 分化チェックポイント機構真核生物 DNAポリメラーゼ Cdc2キナーゼ Cdc25フォスファターゼ哺乳動物 cdk4 ONAポリメラーゼα 減数分裂

项目摘要

The goal of this research is to understand the molecular mechanism controlling the cell cycle "start" and the cell differentiation "start". To this end, fission yeast has been studied as a model organism because this organism is closely related to higher eukaryotes. During the past 3 years, we have discovered 4 novel factors controlling the onset of differentiation and 5 new factors controlling the cell cycle "start". Among the 4 factors are a B-type cyclin that negatively controls the onset of differentiation and positively controls the start of the cell cycle ; a stress signal MAP kinase called Phh1 that positively controls the onset of differentiation ; a novel protein named Rcd1 that is essential for nitrogen-starvation-induced start of differentiation ; Nrd1, a RNA binding protein that negatively controls the onset of differentiation. They have mammalian homologues, raising the possibility that the differentiation control system found in fission yeast may be conserved up to mammals.Among the 5 cell cycle "start" factors are Res2, a DNA binding subunit that forms a transcriptional factor complex with Cdc10 ; Rep1 and Rep2, two transcriptional activator subunits that act by forming a complex with Res2-Cdc10 ; the new cyclin Pas1 that associates with Cdc2 kinase and activates Res2/Cdc10/Rep2 ; Spt1 a novel factor that binds Cdc18 and critically controls the onset and progression of S phase. Spt1 might form a control cascade independent of the Cdc2/Pas1-Res/Cdc10/Rep-Cdc18 cascade.

本研究的目的是了解控制细胞周期“启动”和细胞分化“启动”的分子机制。为此，由于分裂酵母与高等真核生物密切相关，因此被作为模式生物进行研究。在过去的3年里，我们发现了4个控制分化开始的新因素和5个控制细胞周期“开始”的新因素。在这4个因子中，有一种B型细胞周期蛋白，它负向控制分化的开始，并积极控制细胞周期的开始；一种名为Phh1的应激信号图激酶，它积极控制分化的开始；一种名为Rcd1的新蛋白，它对氮饥饿诱导分化的开始至关重要；Nrd1，一种负向控制分化开始的RNA结合蛋白。在5个细胞周期的“起始”因子中，包括与CDC10形成转录因子复合体的dna结合亚基Res2；与Res2-CDc10形成复合体的两个转录激活亚基Rep1和Rep2；与CDc2激酶结合并激活Res2/CDc10/Rep2的新的细胞周期蛋白Pas1；与CDc18结合并关键控制S期发生和发展的新因子Spt1。Spt1可能形成独立于Cdc2/Pas1-res/Cdc10/Rep-Cdc18级联的控制级联。