Development of methods for using fission yeast as a test tube for analyzing highly complex biological systems

我们开发了以下方法来利用分裂酵母作为活的试管来研究哺乳动物高度复杂的生物系统。所开发的方法包括：1)高效的裂解酵母转化方法；2)高效的酵母表达载体；3)构建哺乳动物表达文库和在裂解酵母中转导的高效载体；4)分离可用作同源重组基因替换靶点的非必需基因。这些基因包括：1)哺乳动物的Min1基因；2)哺乳动物的Wos A、B基因；3)哺乳动物的CDC25A磷酸酶基因；4)分裂酵母的REPL基因；5)分裂酵母的Resl基因；6)酵母的CDSL基因。

We developed the following methods for utilizing fission yeast as a live test tube to examine mammalian highly complex biological systems. The methods developed include : 1)a highly efficient fission yeast transformation method ; 2)efficient yeast vectors for transducing cDNA expression libraries ; 3)an efficient vector for construction of mammalian expression cDNA libraries and transduction in fission yeast ; 4)isolation of non-essential genes that can e used for target sites for gene replacement by homologous recombination.By using the developed methods, we succeeded in isolation of very important cell cycle control genes from mammals as well as fission yeast. They include : 1)Minl gene from mammals ; 2)Wos A,B genes from mammals ; 3)Cdc25A Phosphatase gene from mammals ; 4)repl from fission yeast ; 5)resl from fission yeast ; 6)cdsl from yeast.