Development of methods for using fission yeast as a test tube for analyzing highly complex biological systems

我们开发了以下方法，用于利用裂变酵母作为活试管来检查哺乳动物高度复杂的生物系统。所开发的方法包括：1）高效的裂殖酵母转化方法，2）用于转导cDNA表达文库的高效酵母载体，3）用于构建哺乳动物表达cDNA文库并在裂殖酵母中转导的高效载体，4）用于构建哺乳动物表达cDNA文库并在裂殖酵母中转导的高效载体。4）分离非必需基因，所述非必需基因可用于通过同源重组进行基因置换的靶位点。通过使用所开发的方法，我们成功地从哺乳动物和裂殖酵母中分离出非常重要的细胞周期控制基因。它们包括：1）来自哺乳动物的Minl基因; 2）来自哺乳动物的Wos A、B基因; 3）来自哺乳动物的Cdc 25 A磷酸酶基因; 4）来自裂殖酵母的repl; 5）来自裂殖酵母的resl; 6）来自酵母的case 3。

We developed the following methods for utilizing fission yeast as a live test tube to examine mammalian highly complex biological systems. The methods developed include : 1)a highly efficient fission yeast transformation method ; 2)efficient yeast vectors for transducing cDNA expression libraries ; 3)an efficient vector for construction of mammalian expression cDNA libraries and transduction in fission yeast ; 4)isolation of non-essential genes that can e used for target sites for gene replacement by homologous recombination.By using the developed methods, we succeeded in isolation of very important cell cycle control genes from mammals as well as fission yeast. They include : 1)Minl gene from mammals ; 2)Wos A,B genes from mammals ; 3)Cdc25A Phosphatase gene from mammals ; 4)repl from fission yeast ; 5)resl from fission yeast ; 6)cdsl from yeast.