Development of methods for using fission yeast as a test tube for analyzing highly complex biological systems

我们开发了以下方法，利用裂变酵母作为活试管来检查哺乳动物高度复杂的生物系统。所开发的方法包括：1)高效的裂变酵母转化法；2)高效酵母载体转导cDNA表达文库；3)构建哺乳动物表达cDNA文库并在裂变酵母中转导的高效载体；4)分离可用于同源重组基因替代靶位的非必需基因。利用所开发的方法，我们成功地从哺乳动物和裂变酵母中分离出了非常重要的细胞周期控制基因。它们包括：1)没有哺乳动物基因；2)Wos A、B基因来自哺乳动物；3)哺乳动物Cdc25A磷酸酶基因；4)裂变酵母的反应；5)裂变酵母的产物；6)来自酵母的cdsl。

We developed the following methods for utilizing fission yeast as a live test tube to examine mammalian highly complex biological systems. The methods developed include : 1)a highly efficient fission yeast transformation method ; 2)efficient yeast vectors for transducing cDNA expression libraries ; 3)an efficient vector for construction of mammalian expression cDNA libraries and transduction in fission yeast ; 4)isolation of non-essential genes that can e used for target sites for gene replacement by homologous recombination.By using the developed methods, we succeeded in isolation of very important cell cycle control genes from mammals as well as fission yeast. They include : 1)Minl gene from mammals ; 2)Wos A,B genes from mammals ; 3)Cdc25A Phosphatase gene from mammals ; 4)repl from fission yeast ; 5)resl from fission yeast ; 6)cdsl from yeast.