Identification of receptors for insect neuropeptides

昆虫神经肽受体的鉴定

• 批准号: 06454081
• 负责人: KATAOKA Hiroshi
• 金额: $ 1.92万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454081/
• 关键词: insect; rceptor; Bombyxin; insulin; PTTH; Bombyx; 昆虫ホルモン

1. An Bombyx cell line, named BM-N4, has a single class of receptors for bombyxin with a Kd=2.4nM and each cell has 16,000 binding sites on the cell surface. The bombyxin receptor was composed of a several subunits linked with disulfide bonds and the molecular weight of binding subunit was about 105kDa. In addition, an about 92kDa protein was phosphorylated on tyrosine residue immediately after adding bombyxin.2. The nucleotide sequence of the cDNA for bombyxin receptor from BM-N4 cells was elucidated by using PCR assisted method. The deduced amino acid seqence revealed that bombyxin receptor is similar to insulin receptor and composed of two subunits.3. The prothoracic glands have a single class of receptors for PTTH with a Kd=54 pM and each cell has 16,000 binding sites on the cell surface. The molecular weight of PTTH receptor showed about 75kDa by cross-linking experiment.

1. BM-N4是一种家蚕毒素受体，其Kd值为2.4 nM，每个细胞表面有16，000个结合位点。家蚕毒素受体由多个亚基组成，亚基之间通过二硫键连接，结合亚基的分子量约为105 kDa。另外，加入家蚕毒素后，在酪氨酸残基上立即有一个约92 kDa的蛋白质被磷酸化.采用PCR辅助方法，从BM-N4细胞中扩增出家蚕毒素受体cDNA的核苷酸序列。推导的氨基酸序列显示，家蚕毒素受体与胰岛素受体相似，由两个亚基组成.前胸腺具有单一类别的PTTH受体，Kd=54 pM，每个细胞在细胞表面上具有16，000个结合位点。交联实验表明PTTH受体的分子量约为75 kDa。

项目成果

T.Ichikawa et al.: "Functional dibberentiation 〜 bmbyx ineri" Zool.Sci. 13. 21-25 (1996)

T.Ichikawa 等人：“功能分离 ~ bmbyx ineri”Zool.Sci. 13. 21-25 (1996)

T.Ichikawa et al.: "Functional differentiation of neurosecretory cells with immunoreactive diapause hormone and pheromone biosynthesis activating neuropeptide of the moth,Bombyx mori" Zool.Sci.13. 21-25 (1996)

T.Ichikawa 等人：“神经分泌细胞与免疫反应性滞育激素和信息素生物合成激活蛾（Bombyx mori）神经肽的功能分化”Zool.Sci.13。

M.Iwami et al.: "Bombyxire-related peptides:cDNA structure and expression in the brain of hornworm Agrius convoluvoli" Insect Biochem.Mol.Biol.26. 25-32 (1996)

M.Iwami 等人：“Bombyxire 相关肽：Agrius convoluvoli 角虫大脑中的 cDNA 结构和表达”Insect Biochem.Mol.Biol.26。

M.Iwami et. al.: "Bombynin - related peptides:cRNA structure and experssion in the brain of hornuiorm Aguius convoluvoli" Insect Biochem mol.Bil.26. 25-32 (1996)

M.Iwami 等。

M.Iwami et al.: "Bombyxin-related peptides : cDNA structure and expression in the brain of hornworm Agrius convoluvoli." Insect Biochem.Mol.Biol.26. 25-32 (1996)

M.Iwami 等人：“家蚕素相关肽：cDNA 结构和在旋花天蛾大脑中的表达”。