Dynamics and enviromental response of insect hormones controling metamorphosis and diapause

昆虫激素控制变态和滞育的动态和环境响应

• 批准号: 08276101
• 负责人: KATAOKA Hiroshi
• 金额: $ 173.76万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08276101/
• 关键词: insect; silkworm; hormone; ecdysone; metamorphosis; diapause; cis-element; 遺伝子発現; ペプチドホルモン; 前胸腺刺激ホルモン; PBAN; ボンビキシン; 受容体; 休眠ホルモン

Iwami et al. found that bombyxin gene is composed of 30 copies of related genes and identified a specific cis element for bombyxin-producing cells. Ichikawa et al. succeeded in monitoring electrical excitation of neurosecretory cells for 2-5 days. The electrical excitation of these cells producing the same neuropeptide was synchronized and regulated by other central neurons. Yamashita et al. identified a cis element composed of 650 bp for diapause hormone gene by using transgenic fruit fly, Drosophila. Shimada et al. identified gene loci of period, EcR and E75 genes. Aizono et al. found that prothoraicotropic hormone (PTTH) release regulated by serotonin is very important for arousal of sweet potato hornworm from pupal daiapause. Mizoguchi et al. developed a method to quantify a minute amount of PTTH and measured developmental changes of PTTH titer in hemolymph of Bombyx mori. Kataoka et al. identified a candidate of PTTH receptor cDNA by using expression cloning method. The receptor is belong to a family of G-protein coupled receptor. Fujiwara et al. found that expression levels of early late gene, BHR and late gene, urbain were very low in the wingless mutant of Bombyx mori (fl). Yaginuma et al. identified 7 isoforms of EcR.

Iwami等发现，家蚕毒素基因由30个拷贝的相关基因组成，并鉴定了一个产家蚕毒素细胞的特异性顺式元件。Ichikawa等人成功地监测了神经分泌细胞的电兴奋2-5天。这些产生相同神经肽的细胞的电兴奋被其他中枢神经元同步和调节。Yamashita等利用转基因果蝇Drosophila鉴定了一个由650 bp组成的滞育激素基因顺式元件。Shimada等鉴定了period、EcR和E75基因的基因位点。Aizono等发现，由5-羟色胺调节的促胸激素（PTTH）的释放对甘薯天蛾蛹滞育的唤醒非常重要。Mizoguchi等人开发了一种定量微量PTTH的方法，并测量了家蚕血淋巴中PTTH滴度的发育变化。Kataoka等利用表达克隆方法鉴定了PTTH受体cDNA的候选者。该受体属于G蛋白偶联受体家族。Fujiwara等发现早迟基因BHR和迟基因urbain在家蚕无翅突变体中的表达水平很低。Yaginuma等人鉴定了EcR的7种亚型。

项目成果

Y.Shirai: "Carbachol-induced protein phosphorylation in the brain-corpus allatum complex of the silkworm,Bombyx mori." Appl.Entomol.Zool.32・4. 573-581 (1997)

Y. Shirai：“家蚕脑-软体复合体中卡巴胆碱诱导的蛋白质磷酸化。Appl.Entomol.Zool.32·4（1997）”

Masataka Suzuki: "Absence of dosage compensation at the transcription level of a sexlinked gene in a female heterogametic insect,Bombyx mori." Heredity. (in press). (1998)

Masataka Suzuki：“雌性异配昆虫家蚕的性连锁基因的转录水平缺乏剂量补偿。”

Fujiwara H.: "Developmental profiles of wing imaginal discs of flugellos(f1),a wingless mutant of the silkworm,Bombyx mori." Dev.Genes Evol.207. 12-18 (1997)

Fujiwara H.：“flugellos（f1）翅成虫盘的发育概况，flugellos（f1）是蚕的无翅突变体，Bombyx mori。”

Takahashi: "A new family of site-specific retrotransposons,SART1,is inserted into telomeric repeats of the silkworm,Bombyx mori." Nucl.Acids Res.25. 1578-1584 (1997)

Takahashi：“一个新的位点特异性逆转录转座子家族，SART1，被插入家蚕（Bombyx mori）的端粒重复序列中。”

S.-D.Ha: "Structure determination and cDNA cloning of paralytic peptide in Bombyx mori." Peptide Chemistry. (in press).

S.-D.Ha：“家蚕麻痹肽的结构测定和 cDNA 克隆。”