Investigation of G protein receptor signaling for regenerative medicine.

探讨G蛋白信号在胚胎发育和血管生成中的作用。我们能够得到以下结果。为了抑制各种细胞类型的Gi信号，我们开发了一种Cre去除STOP磁带后能表达百日咳的小鼠系。该细胞系用于特异性阻断血管或神经系统中的Gi信号。我们发现Gi对心脏的心内膜-间质转化至关重要。在神经系统中，Gi是神经上皮细胞神经管闭合所必需的。迁移神经元中Gi信号的消融表明除了CXCR4.2外，还存在重要的gpcr。作为G蛋白信号传导的下游效应者，我们聚焦了RhoJ。敲除RhoJ或内皮特异性过表达表明，RhoJ是内皮形态改变所必需的。

To utilize G protein signaling apparatus in regenerative medicine roles of G protein signaling during embryonic development and angiogenesis were explored. We were able to obtain following results.1. To inhibit Gi signaling in various cell types a mouse line that can express pertussi after removal of STOP cassette by Cre was developed. This line was used to block Gi signaling specifically in the vascular or nervous system. We found that Gi is critical for endocardial-mesenchymal transition in heart. In the nervous system Gi is required for neural tube closure in neuroepithelal cells. Ablation of Gi signaling in migrating neurons indicated the existence of important GPCRs in addition to CXCR4.2. As a downstream effecter of G protein signaling we focused RhoJ. Knocking out RhoJ or endothelial-specific overexpression revealed that RhoJ is required for the endothelial morphology change.