健常者・慢性膵炎患者の膵液中抗菌活性の検討と抗菌活性物質の分離精製

健康人和慢性胰腺炎患者胰液抗菌活性的调查及抗菌活性物质的分离纯化

• 批准号: 06770402
• 负责人: 吉田 憲司
• 金额: $ 0.58万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06770402/
• 关键词: 抗菌活性; 膵炎; 慢性膵炎

実験1.膵液中抗菌活性の検討健常者および慢性膵炎患者に対し、セクレチン試験を施行し得られた膵液に、E.coli ATCC 25922を接種後、振盪培養を行い、経時的に生菌数を測定した。その結果、健常膵液では抗菌活性がみられたが、慢性膵炎患者膵液では抗菌活性は認められなかった。これらの結果より、慢性膵炎患者では、E.coliが逆行性に膵管内に進入した際に、それを防ぎにくく、進入した細菌が膵管内に常在化する可能性が示唆された。実験2.抗菌活性物質の分離精製健常膵液を、分子量10,000、1,000の限外濾過膜を用いて精製した。分子量10,000以上、1,000-10,000、1,000以下の各分画にE.coli ATCC 25922を接種後、振盪培養を行い、経時的に生菌数を測定した。その結果、分子量10,000以上および分子量1,000以下の分画の膵液では、E.coli発育の抑制は認められず、分子量1,000-10,000の分画の膵液に抗菌活性が認められた。実験3.慢性膵炎患者膵液の抗菌活性の検討慢性膵炎患者膵液を用いて、実験2.と同様の実験を行った。その結果、慢性膵炎患者膵液には、濃縮前には認められなかった抗菌活性が、精製により、健常膵液と同じく、分子量1,000-10,000の分画に抗菌活性が認められた。

I don't know. If the antimicrobial activity in the liquid is normal, the patients with chronic inflammation should be tested. After receiving E.coli ATCC 25922, the bacterial count of the patients with chronic inflammatory disease should be measured. The results showed that the antibacterial activity of Jianchang liquid was significantly higher than that of patients with chronic inflammatory disease. The results showed that the patients with chronic inflammatory disease, the patients with chronic inflammatory disease, No, no, no. The antimicrobial activity was isolated from the essence, the molecular weight was 10000, and the molecular weight was 1000 nm. The number of living bacteria in each sub-drawing of E.coli ATCC 25922 with molecular weight above 10000, 1000-10000 and below 1000 was measured. The results showed that the molecular weight was more than 10000 and the molecular weight was less than 1000. The antibacterial activity of the solution was confirmed by the inhibition of E.coli, and the antibacterial activity of the solution with molecular weight of 1000-10000. No. 3. The antimicrobial activity of the fluid of patients with chronic inflammatory disease and the fluid of patients with chronic inflammation were used. I'm sorry to hear the same story. The results showed that the fluid samples of patients with chronic inflammatory disease were tested, and the results showed that the antibacterial activity of the patients with chronic inflammatory disease was determined, and the results showed that the antibacterial activity of the patients with chronic inflammatory disease was determined. The results showed that the antibacterial activity of the patients with chronic inflammatory disease was detected by Elisa, and the results showed that the antibacterial activity of the patients with chronic inflammatory disease was different from that of the patients with chronic inflammatory disease, and the molecular weight was 1000-10000.