Japan-France Joint Study on Development in Higher Plants

日法高等植物开发联合研究

• 批准号: 07044178
• 负责人: KAMADA Hiroshi
• 金额: $ 7.3万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044178/
• 关键词: Higher Plants; Embryogenesis; Phytohormones; Signal Transducton; Control of Gene Expresson; Functional Differentiation; Molecular Mechanisms; Sexuality; 機能分化; 花芽形成; 光形態形成; 遺伝子操作

During 3 years of this project, 16 Japanese and 3 French researchers visited each country, and the following joint-researchers have been done.1. Metabolism of Phytohormones : Using various Arabidopsis mutants obtained in France, genes mediating the synthesis of auxin and abscisic acid (ABA) were newly isolated. 2. Embryogenesis and Regulation of Gene Expression : Using a transcription factor mediating embryo-specific ABA signal transduction which was isolated in France, a homologous gene of carrot was isolated. We made transgenic plants and cells in which the homologue was over-expressed and characterized it. We succeeded to identify some genes of which the expresson was controlled by the transcription factor, and moreover, it was clarified that the transcription factor and the down-regulated genes are involving in the acquisition of desiccattion tolerance. On the other hand, using the antibody against calmodulin-binding proteins in carrot which was prepared in France, the cahnge of th … More e protein patterns during carrot somatic embryogenesis was identified. 3. Flower Differentiation and Sexuality : When Japanese researchers isolated a Pharbitis gene of which the expression was detected just after the flower-inductive photoperiodic treatment and compared the DNA sequence to known genes, it was found that the gene shows strong similarity to one of the genes which were isolated as EST clones in Arabidopsis seeds in France. By exchange of the informations of the genes obtained in Japan and France, we conducted to compare the physiological functions of the genes and the characteristics of the expression and finally the overall images of the gene family was clarified. Furthermore, using Cannabis in Japan and Melandryum in France, we succeeded to isolate and characterize a part of sex chromosomes in both plants. 4. Cell Division : Studies on new peptide growth factors stimulating plant cell division have been proceeded, and a new aspect of cell division in plants was opened.The Japan-French Workshop on "Plant Physiology and Plant Molecular Biology" was held in Tsukuba city in the autumn of 1997. In the workshop, Japan and French Researchers concerning with this Joint-Program presented their results obtained in this co-operative work and discussed enough the research and the future collaboration. Less

在该项目的3年中，16名日本研究人员和3名法国研究人员访问了每个国家，并进行了以下联合研究。植物激素的代谢：利用在法国获得的各种拟南芥突变体，新分离出介导生长素和脱落酸（阿坝）合成的基因。2.胚胎发生与基因表达调控：利用法国分离的一个介导胚特异性阿坝信号转导的转录因子，克隆了胡萝卜的一个同源基因。我们获得了过量表达该同源基因的转基因植株和转基因细胞，并对其进行了鉴定，成功地鉴定了一些受转录因子调控表达的基因，进一步阐明了转录因子和下调表达的基因参与了植物脱水耐性的获得。另一方面，利用法国制备的抗胡萝卜钙调素结合蛋白抗体， ...更多信息 鉴定了胡萝卜体细胞胚胎发生过程中的蛋白质类型。3.花的分化和性：当日本研究人员分离出一个牵牛属的基因，在花诱导光周期处理后不久就检测到其表达，并将其DNA序列与已知基因进行比较时，发现该基因与法国拟南芥种子中分离出的EST克隆基因之一具有很强的相似性。通过对日本和法国获得的基因信息的交换，对基因的生理功能和表达特点进行了比较，最终阐明了基因家族的整体形象。此外，利用日本的大麻和法国的Melandryum，我们成功地分离和表征了这两种植物的性染色体的一部分。4.细胞分裂：促进植物细胞分裂的新肽类生长因子的研究正在进行，为植物细胞分裂开辟了新的领域。1997年秋，在筑波市召开了日法“植物生理学和植物分子生物学”研讨会。在研讨会上，日本和法国的研究人员介绍了他们在这项合作工作中取得的成果，并充分讨论了研究和未来的合作。少

项目成果

N.Kawakami: "ABA insensitivity and low ABA levels during seed development of non-dormant wheat mutants." J.Exp.Bot.48. 1415-1421 (1997)

N.Kawakami：“非休眠小麦突变体种子发育过程中 ABA 不敏感且 ABA 水平较低。”

鎌田博: "植物生理学分野における日仏ワークショップを開催して" 植物の化学調節. 31. 80-82 (1996)

Hiroshi Kamata：“法国-日本植物生理学研讨会”植物化学调节。31. 80-82 (1996)

M.Umeda: "Melecular and biochemical characterization of a subunit of the proteasome in rice and carrot cells." Molecular General Genetics. (in press). (1997)

M.Umeda：“水稻和胡萝卜细胞中蛋白酶体亚基的分子和生化特征。”

H.Sekimoto, et al.: "Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana" Plant Cell Physiol.39 ; (in press). (1998)

H.Sekimoto 等人：“拟南芥中醛氧化酶的分子克隆和表征”Plant Cell Physiol.39；

C. Ooura: "Preparation and detection of calmodulin binding proteins using biotinylated calmodulin." Asia-Pacific Journal of Molecular Biology and Bio-technology,. 5. 61-63 (1997)

C. Ooura：“使用生物素化钙调蛋白制备和检测钙调蛋白结合蛋白。”