Studies on cultivation and utilization of transgenic plants in open field

在本研究中，我们进行了两种类型的实验。第一个是在基础和应用领域分离和鉴定生产有价值的转基因植物的有用基因。第二步是在室内(室内实验)和半封闭温室(室外第一步)培养转基因植株，根据重组DNA实验指南，对转基因植株的遗传类型、生理特性分析以及对周围环境的影响等性状进行评价。我们成功地分离并鉴定了与抗虫性相关的新型蛋白酶抑制基因、类细菌蛋白(GLP)和PnC401基因，以及与光周期诱导开花相关的各种光敏和隐色素基因、性染色体特异基因等。其中，GLP和PnC401与内源生物钟的关系备受关注。更进一步的…此外，过量表达GLP的转基因烟草在非诱导开花的光周期条件下表现出早花现象，被认为可以用于人工控制开花。将Ri-rol基因、卡那霉素抗性基因、GUS基因等导入农作物和园艺植物中，并对转基因植株进行了培养和分析。其中，在半封闭式(P1)温室条件下对高表达RolC基因的颠茄进行了详细的栽培和分析。对导入的转基因和转基因衍生性状进行孟德尔遗传，并对其稳定性进行了验证。此外，在P1温室条件下，转基因颠茄的花粉散布、异花授粉能力、对土壤细菌的影响、成草可能性、冬季生活力、生物碱产量等特性均与非转基因颠茄相当。利用这些结果，我们对指南进行了修改，并支持了东北大学开放实验场(户外实验第二步)的建立。较少

In this research, we conducted two types of experiments. The 1st one is isolation and characterization of useful genes for production of valuable transgenic plants in basic and applied fields. The 2nd one is to cultivate transgenic plants in experiment room (in-door experiment) and then in semi-closed greenhouse (1st step of out-door experiment) for evaluating characters of transgenic plants, such as types of inheritance, analyses of physiological characteristics, and effects on its surrounding environments, according to the guideline for recombinant DNA experiments.We succeeded to isolate and characterize a new type of proteinase inhibitor gene which is thought to be relating to insect resistance, germin-like protein (GLP) and PnC401 genes which are relating to photoperiodic induction of flowering, various types of phytochrome and cryptochrome genes, sex-chromosome specific genes and others. Among them, GLP and PnC401 were taken notice in relation to endogenous biological clock. Furth … More ermore, transgenic tobacco over-expressing GLP showed early flowering under a non-inductive photoperiodic condition for flowering and is thought to be utilizable in artificial control of flowering.The Ri-rol genes, kanamycine-resistant gene, GUS gene and others were introduced in crops and horticultural plants and the transgenic plants were cultivated and analyzed. Among them, transgenic belladonna (Atropa belladonna) over-expressing rolC gene were cultivated and analyzed in detail in semi-closed (P1) greenhouse. The introduced transgene and the transgene-derived characters were inherited by Mendelian fasion and we confirmed the stabilities. Moreover, in P1 greenhouse condition, the characters of the transgenic belladonna (pollen dispersion, cross-pollination ability, effects on soil bacteria, possibility for becoming weeds, viability in winter season, productivity of alkaloid and others) were confirmed to be comparable to those of non-trangenic one. Using these results, we made the modification of guideline and supported the establishment of open experimental field (2nd step of out-door experiment) in Tohoku University. Less