Study on the interaction of epigenetic factors and regulation of gene expression in higher plant embryogenesis

为了评估组蛋白脱乙酰酶(HDAC)抑制剂诱导的萌发后生长抑制是否在高等植物中普遍存在，对3个拟南芥近缘物种进行了曲古抑素A处理。结果表明，向营养生长期的转变受到了抑制，这表明HDAC普遍参与了这一转变。另一方面，C-LEC1基因的表达和C-LEC1上游DNA甲基化水平在胡萝卜胚胎诱导的早期出现高峰。DNA甲基化的短暂增加来自CxG和CHH环境，可能由从头类型甲基酶控制。

In order to evaluate whether the inhibition of growth after germination induced by histone deacethylase (HDAC) inhibitors was a universal phenomenon in higher plants, Trichostatin A treatment was performed to three Arabidopsis related species. As a result, transition to the vegetative phase was inhibited in all, and it was suggested that HDAC is involved in this transition universally. On the other hand, expression of C-LEC1 gene and DNA methylation level in upstream region of C-LEC1 were observed peaks in early timing of embryo induction in carrot. The transiently increasing of DNA methylation was derived from CxG and CHH contexts that might be controlled by de novo type methylase.