Study on the interaction of epigenetic factors and regulation of gene expression in higher plant embryogenesis

高等植物胚胎发生中表观遗传因子相互作用及基因表达调控研究

• 批准号: 23570045
• 负责人: KAMADA Hiroshi
• 金额: $ 3.49万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570045/
• 关键词: 胚発生; エピジェネティクス; 成長相転換

In order to evaluate whether the inhibition of growth after germination induced by histone deacethylase (HDAC) inhibitors was a universal phenomenon in higher plants, Trichostatin A treatment was performed to three Arabidopsis related species. As a result, transition to the vegetative phase was inhibited in all, and it was suggested that HDAC is involved in this transition universally. On the other hand, expression of C-LEC1 gene and DNA methylation level in upstream region of C-LEC1 were observed peaks in early timing of embryo induction in carrot. The transiently increasing of DNA methylation was derived from CxG and CHH contexts that might be controlled by de novo type methylase.

为了评估组蛋白脱乙酰酶(HDAC)抑制剂诱导的萌发后生长抑制是否在高等植物中普遍存在，对3个拟南芥近缘物种进行了曲古抑素A处理。结果表明，向营养生长期的转变受到了抑制，这表明HDAC普遍参与了这一转变。另一方面，C-LEC1基因的表达和C-LEC1上游DNA甲基化水平在胡萝卜胚胎诱导的早期出现高峰。DNA甲基化的短暂增加来自CxG和CHH环境，可能由从头类型甲基酶控制。

项目成果

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シロイヌナズナおよび近縁野生種におけるヒストン脱アセチル化酵素阻害剤Trichostatin Aによる胚発生関連転写因子の発現の相違

组蛋白脱乙酰酶抑制剂曲古抑菌素 A 诱导拟南芥及相关野生物种胚胎发育相关转录因子表达的差异

• 发表时间: 2012
• 作者: 島田尚久;針金谷尚人;鎌田博
• 通讯作者: 鎌田博