Study on the interaction of epigenetic factors and regulation of gene expression in higher plant embryogenesis

为了评价组蛋白去乙酰化酶（HDAC）抑制剂对植物萌发后生长的抑制是否是高等植物中的普遍现象，对3种拟南芥属植物进行了曲古抑菌素A（Trichostatin A）处理。结果表明，向营养生长期的过渡在所有情况下都受到抑制，这表明HDAC普遍参与了这种过渡。另一方面，C-LEC 1基因的表达和C-LEC 1上游区域的DNA甲基化水平在胡萝卜胚胎诱导的早期出现高峰。DNA甲基化的短暂增加可能是由CxG和CHH背景引起的，可能是由从头甲基化酶控制的。

In order to evaluate whether the inhibition of growth after germination induced by histone deacethylase (HDAC) inhibitors was a universal phenomenon in higher plants, Trichostatin A treatment was performed to three Arabidopsis related species. As a result, transition to the vegetative phase was inhibited in all, and it was suggested that HDAC is involved in this transition universally. On the other hand, expression of C-LEC1 gene and DNA methylation level in upstream region of C-LEC1 were observed peaks in early timing of embryo induction in carrot. The transiently increasing of DNA methylation was derived from CxG and CHH contexts that might be controlled by de novo type methylase.