Japan-French joint study in plant physiology based ongenome

本研究是由具有丰富研究经验的日本和法国研究人员合作，利用拟南芥、水稻等植物的多种基因组信息和多种突变体，对高等植物的一些重要生理问题进行研究。本项目主要研究方向为胚胎发生、维管形成、花诱导、自交不亲和等，特别是细胞分裂、细胞结构、细胞死亡等细胞事件是植物个体生理反应的功能基础，以及调节植物激素（生物合成和信号转导）、生物钟等现象的成分。此外，考虑到两国转基因植物的实际问题，还对转基因作物、产生有用次生代谢物的转基因植物、用于植物修复的转基因植物等转基因植物的研究进行了研究，因为这些研究应用广泛。在基础研究的基础上，国际上正在进行更多的研究，这也是本项目的主要课题。本项目取得的研究成果如下：脱落酸（ABA）生物合成限速酶基因的分离与表征、ABA信号转导控制因子的分离与表征、胚胎特异性基因的分离与表征及其表达机制、植物激素（生长素、细胞分裂素和油菜素内酯）诱导基因与生理现象的关系、控制胚胎发生、维管形成的遗传程序主要因子的鉴定。光周期诱导开花等形态发生，转基因作物检测新方法等。2001年秋，在法国召开了日法研讨会，参与该项目的多名日本和法国研究人员参加了研讨会，讨论了各自的研究成果，并对未来日法在植物生理学方面的合作进行了咨询。少

This research had been conducted to study some important physiological problems in higher plants, in collaboration of researchers of Japan and France who had many research experience in their collaboration, using various genome information and many mutants of Arabidopsis, rice and other plants. Main subjects in this project were embryogenesis, vascular formation, flower induction, self-incompatibility, and others, especially cellular events like cell division, cell structure, cell death and others which are functional base of physiological responses in individual plants, and components regulating the phenomena like phytohormones (biosynthesis and signal transduction), biological clock and others. Moreover, studies on transgenic plants like transgenic crops, transgenic plants producing useful secondary metabolites, transgenic plants for phytoremediation were also investigated as considering practical problems on the genetically modified plants in both countries, because these applied re … More searches are carrying out in the world based on basic research which were main subjects in this projectDuring 3 years from 1999 to 2001, many research outcomes were obtained in this project as follows ; isolation and characterization of a gene encoding a rate limiting enzyme of abscisic acid (ABA) biosynthesis, isolation and characterization of factors controlling ABA signal transduction, isolation and characterization of embryo-specific genes and the mechanisms of its gene expression, relation between phytohormone (auxin, cytokinin and brassinosteroid)-inducible genes and physiological phenomena, identification of main factors in genetic program controlling embryogenesis, vascular formation, photoperiodic induction of flowering, and other morphogenesis, a new method for detection of transgenic crops, and others. In the autumn of 2001, Japan-French workshop was held in France and many researchers of Japan and France who participated in this project attended it, discussed on their research outcomes and consulted the future collaborative works between Japan and France in plant physiology. Less